1 / 18

INTERACTION BETWEEN TUMOR & ITS MICROENVIRONMENT

INTERACTION BETWEEN TUMOR & ITS MICROENVIRONMENT. AVNER FRIEDMAN. Mathematical Biosciences Institute Ohio State University, Columbus, USA. Thin slice of a breast tumor. Background on Breast Cancer. http://www.jamesline.com/images/frontiers/summer2005/PartnersInCrime1.jpg.

devlin
Download Presentation

INTERACTION BETWEEN TUMOR & ITS MICROENVIRONMENT

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. INTERACTION BETWEEN TUMOR & ITS MICROENVIRONMENT AVNER FRIEDMAN Mathematical Biosciences Institute Ohio State University, Columbus, USA

  2. Thin slice of a breast tumor Background on Breast Cancer http://www.jamesline.com/images/frontiers/summer2005/PartnersInCrime1.jpg ● Epithelial cells are transformed by gene mutations (TECs). ● TECs secrete TGF-ß (transforming growth factor beta). ● TGF-ß signals to fibroblasts which get transformed to myofibroblasts; both secrete EGF (epidermal growth factor). ● Fibroblasts/myofibroblasts form the microenvironment of the tumor ● We want to examine how they interact with the TECs at the very early stage of cancer.

  3. Complex pathways stimulating cell proliferation EGF, RAS, MAPK TGF-b, SMAD biocarta.com

  4. TRANSFORMED EPITHELIAL CELLS (TECs)

  5. fibroblasts myofibroblasts EXPERIMENT & MODEL DOMAIN MEMBRANE ( TECs ) ● Place TECs in solution at bottom of well. ● Place semi-permeable membrane above the solution. ● Deposit fibroblasts/myofibroblasts above the membrane. ● Count the number of TECs in day 2, 4, 6, and 8. ● TGF-ß and EGF can cross the membrane, but cells cannot. MEMBRANE - + fibroblasts ( f ) TEC ( n ) myofibroblasts ( m ) EGF (E) EGF (E) TGF-b (G) TGF-b (G) X = 0

  6. Diffusion of TECs

  7. CHEMOTAXIS : n cells move towards increasing concentration of EGF

  8. Cell proliferation : logistic growth enhanced by EGF via Hill-type kinetics

  9. production of EGF by fibroblasts & myofibroblasts in -

  10. on the semi-permeable membrane

  11. ● Having gained understanding of the early stage of breast cancer in vitro without ECM, the next step is to include ECM, using the value of g that was established.

More Related