1 / 8

Microfluidic Multi-Patch Recording Unit

Microfluidic Multi-Patch Recording Unit. Kachi Odoemene Physiological Sensing Facility Purdue University. Patch Clamp Technique . Measure intracellular current from single ion channels of excitable cells Glass micropipette 1-2um in diameter

devin
Download Presentation

Microfluidic Multi-Patch Recording Unit

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. MicrofluidicMulti-Patch Recording Unit Kachi Odoemene Physiological Sensing Facility Purdue University

  2. Patch Clamp Technique • Measure intracellular current from single ion channels of excitable cells • Glass micropipette 1-2um in diameter • Giga-ohm seal formation between glass electrode and cell membrane Closed Open Glass electrode Ion channel Cell Membrane Hamil, O et al 1981 Neher and Sakman 1992

  3. Limitations of Traditional Patch • Operation requires high skill level • Low throughput • Approximately 10 cells/day • Impossible to record from population of neurons in local network http://www.farma.ku.dk/uploads/pics/PatchClamp.jpg

  4. Conceptual Design Backside microfluidic channels Circular Patch Site Access port for electrode and suction tubing PDMS chamber

  5. Patch Substrate Design Top side Back side • Double sided processing on either glass or silicon substrate • Backside: Tapered microfluidic channels • From 10 um wide to 500 um wide • Topside: Patch pores and microtubing access port • Holes aligned to mate with with channel • Application dependent array size and spacing Patch pores 1-2 um diameter

  6. Proof of Device Concept in Silicon • Implement chip design using Deep Reactive Ion Etching (DRIE) • Fast etch rate (>8 um/min) • Wet oxidation to create glass-like layer and shrink patch pores • Integration with PDMS SEM micrographs of microchannels Confocal micrograph of narrow channels

  7. Glass Fabrication • High aspect ratio glass DRIE requires hard mask • Thick electroplated nickel (>5 um) • Very slow etch rate • 0.5 um/min • Currently laser micromachining channels and pores Nickel hard mask patterned with photoresist Channels etched via DRIE, with NI hard mask

  8. Potential Commercial Advantages • Overall unique chip design in glass • Embedded microfluidic channels • Isolated studies of channels of single cell or cells in a network • Glass substrate allowing giga-seal formation & electrical isolation • Recordings from adherent cells in tissue culture and cells with native ion channels • Device automation will lead to high throughput drug screening applications

More Related