金颗粒的合成及连接

1. 金颗粒的合成及连接 The synthesis of gold nanoparticles and their linkage Yuxing Ma 2014/4/23

2. The synthesis of gold nanoparticles AuCl3·HCl·4H2O

3. N+—[CH2(CH2)6CH3]4—Br- (toluene)

4. Borohydride solution stirred The two solutions were poured into a 500mL separation funnel with a glass cap and gently shaken for about 5 min until the yellow color disappeared from the aqueous phase and the toluene phase became red colored.

5. Ostwald Ripening—奥斯瓦尔德熟化 是一种可在固溶体（solid solutions）或液溶胶（ liquid sols）中观察到的现象，指其中的非均匀结构随时间流逝所发生的变化：溶质中的较小型的结晶或溶胶颗粒溶解并再次沉积到较大型的结晶或溶胶颗粒上。

7. Left to right: trioctylphosphine oxide (TOPO), triphenylphosphine (TPP), dodecanethiol (DDT), tetraoctylammonium bromide (TOAB) and oleic acid (OA).

8. d=deff-2.2nm

9. dynamic light scattering, DLS

10. The synthesis of polymer Scheme of the synthesis of the amphiphilic polymer and the coating of nanoparticles.

11. The linkage of nanoparticles α-Amino-ω-alkyne PEG α-Amino-ω-azido PEG

12. EDC reaction 1-ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride

14. The isolation method Dialysis bag: 8000~14000 Ultrafiltration tube: 1000,000 MWCO

15. >90%

16. The azide/alkyne functional groups and their conjugation are highly selective and are essentially inert to most biological molecules, oxygen, water, and the majority of common reaction conditions in chemical synthesis, and are tolerant of a wide range of solvents, temperatures, and pH values.

17. Click Chemistry • Click chemistry is a method to generate substances by joining small units together with heteroatom links (C-X-C). That can work reliably in both small- and large-scale applications. • The reaction must be give very high yields, generate only inoffensive byproducts that can be removed easily. • The required process characteristics include simple reaction conditions (ideally, the process should be insensitive to oxygen and water). • Purification(if required)must be by nonchromatographic methods, such as crystallization or distillation. • The product is stable under physiological conditions.

19. Thanks！