the development of cell model and biochip platform
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細胞模型和生物晶片平台的發展 (The development of cell model and biochip platform)

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細胞模型和生物晶片平台的發展 (The development of cell model and biochip platform). Prof. Ching-Hsing Luo National Cheng Kung University Tainan, Taiwan. Cell Model in Ion Channles Luo-Rudy model in 1991 &1994 Circ. Res. Ca 2+. Action Potential by Model Simulation.

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the development of cell model and biochip platform

細胞模型和生物晶片平台的發展(The development of cell model and biochip platform)

Prof. Ching-Hsing Luo

National Cheng Kung University

Tainan, Taiwan

action potential by model simulation
Action Potential by Model Simulation

Luo-Rudy model simulation by Prof. Ching-Hsing Luo

  • A standard procedure of model simulation has been developed to
  • evaluate the accuracy of simulation results
  • 2. A physiological simulation fake is discussed.
international invitation cell attachment for drug screen
International Invitation: Cell Attachment for Drug screen

by Miqin Zhang

High performance of integrated cell-based sensors for drug screening

with impedance measurement technology.

mission
Mission
  • Medical Researches

Stem cell, Neuron cell, Cancer cell for Drug screening, Differentiation or cultivation control, Gene therapy

  • Engineering Technologies

Chip – micro/nano (channel and pumping)

Sensor and actuator – micro/nano scale

Cell Modeling – Ischemia, drug effects

team up
Team Up

Domestic:

  • Cell-based microchip platform –李清庭、羅錦興、魏憲鴻、 鍾宜璋
  • Cell Model - 吳勝男、羅錦興、鄧君豪
  • Bio-application – 宋瑞珍副校長、林茂村院長、吳勝男

International: Univ. of Washington

  • Miqin Zhang: drug screening
  • Meldrum: HIV, AIDS applications
integration structure
Integration Structure

Patch Clamping Microchip Platform with Cell Modeling (Project 3)

Microfluidic Chip

(Project 2)

Single-Cell Microchip Operation Platform (protocol control, cell clamp, and cell model)

Bio-application studies: stem cells, cardiac cells, and neurons

(Project 1)

Common Facilities (Core A)

International and domestic collaboration

cell chip platform

Microfluid Flow

Single Cell

Microelectrode

Micromixer

Microchannel

Microelectrode

Microvalve

Micropump

Microvalve

Flow sensor

1. Automatic Protocol control (concentration clamp)

2. Voltage clamp for ionic channel measurement

Micropump

Cell Chip Platform
passive mixing in microfluidic channel by prof wei
Passive Mixing in Microfluidic Channelby Prof. Wei

q

Channel Width

Setup

Width 300 mm

Open angle q=60

cell clamping microchip platform

Silicon oxide

Differentiation or cultivation protocols

Electrode

Sucking pressure

Silicon

Glass

Cell Clamping Microchip Platform
cell attachment and rejection

Cell

Electrode

Channel

Cell Attachment and Rejection

Cell Rejection Compound

cell attachment implementation 1 by prof chung and prof luo

PDMS

Cell-Attachment (Implementation-1)by Prof. Chung and Prof. Luo
  • Using flat PDMS imprint SH-(CH2)11COOH on Au

cell favoring coating

Imprinting:

Time≒ 60sec

Chemical bond:

SH-(CH2)11COOH

Au

cell attachment implementation 2
Cell-Attachment (Implementation-2)
  • Immerse into DSPC solution (about 12~24 hours)
  • (cell hating)

DSPC

cell

cell attachment result 1 by prof lin prof chung and prof luo
Cell-Attachment (Result-1)by Prof. Lin, Prof. Chung and Prof. Luo

Morphology of the cell on the platform

  • Different geometric platform:

square、circle、ellipse

  • Different size platform

(all are 1.6 μm height)

preparations
Preparations
  • Cell name : human CD34+ Progenitor Cell
  • Diluent : Phosphate Buffered Saline (PBS)
  • Na2HPO4 -------------------------------------- 10.9 g
  • NaH2PO4 -------------------------------------- 3.2 g
  • NaCl ------------------------------------------- 90 g
  • Distilled water --------------------------------- 1000 ml
  • Counterstain :Trypan blue
attachment structure
Attachment Structure

flowing

Platform

(a)Before adhering

cell

1.6 μm

silicon

cell

(b) After adhering

platform

Au on silicon base

electrode shape effect
Electrode Shape Effect
  • Cell is stretched to platform corner
  • μmdiameter
  • circle platform

18x18 μm2

square platform

18x36 μm2

ellipse platform

spherical shape
Spherical Shape

Original platform

cell

Au on silicon base

platform

rectangular shape
Rectangular Shape

Original platform

cell

Au on silicon base

platform

electrode bigger than cell
Electrode bigger than cell
  • Cell may be stretched to platform edge

27x54 μm2

ellipse platform

37μmdiameter

circle platform

37x74 μm2

ellipse platform

38μmdiameter

circle platform

future work
Future Work
  • Microfluidic driving system
  • Voltage Clamp Chip (2-4 mm hole)
  • Protocol control interacts with ionic channel measurement
  • Update Cardiac Cell Model
  • Vascular Epithelial Cell model for Atherogenic analysis
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