Introduction to Postconviction DNA Testing Innocence Network Conference April 19, 2011 – Charlotte, North Carolina. Olga Akselrod Senior Staff Attorney The Innocence Project. Seth Miller Executive Director Innocence Project of Florida. DNA Basics – What Is DNA?.
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Senior Staff Attorney
The Innocence Project
Innocence Project of Florida
Target Region for PCR
What genes are we looking at?
Base Pair mutations in highly variable regions in the DNA
Base pairs: adenine (A) forms a base pair with thymine (T) and guanine (G) forms a base pair with cytosine (C)
We test these regions because they vary greatly from person to person, not because they have a specific genetic purpose
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DNA testing kits test at 13-16 regions (aka “markers” or “loci”)
TO GET A DNA PROFILE, JUST COUNT THE NUMBER OF REPEATS
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At each locus, you have genetic information inherited from your mom and your dad in the form of a number of repeats. The number of repeats are called “alleles”.
D8 14, 14
D21 29, 31
D7 8, 11
CSF 7, 10
D3 15, 15
THO1 8, 9
D13 11, 11
D16 9, 13
D2 21, 22
D19 12, 12
vWA 14, 16
TPOX 9, 9
D18 10, 15
Amel X, Y
D5 12, 12
FGA 20, 23
The probability of a random match is calculated by applying the product rule to the probability of the allele found at each locus
0.195 x 0.075 = 0.0146 = 1 in 68 people (1/.0146)
0.195 x 0.075 x 0.036 = 0.0005 = 1 in 2000 people (1/.0005)
Multiply them ALL together and you get 1 in 594 trillion!
Modified from DNA.gov