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Projectlab. PCR cycle. Novablue cells were used. 94 degrees C 30 seconds 64 degrees C 30 seconds 72 degrees C 1 minute After 25 cycles finish with 72 degrees C for 5 minutes. Picture of agarose gel. Our PCR Product Molecular weight marker.

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pcr cycle
PCR cycle

Novablue cells wereused

  • 94 degrees C 30 seconds
  • 64 degrees C 30 seconds
  • 72 degrees C 1 minute
  • After 25 cycles finish with 72 degrees C for 5 minutes
picture of agarose gel
Picture of agarose gel

Our PCR Product

Molecularweight marker

slide4

Our vector after purification, it’sprettyweakbutit’sthere.

ligation
Ligation

Novablue cells wereused

No colonieswereformed after ligation. Therefore a backup culturemade by Lasse wasused for screening and analysis.

analysis of transformants
Analysis of transformants

ReferencePCR fragment from anothergroup

Ligatedvector, pretransformation. No vector is visible.

Screening of Lasses colonies, you canclearlysee the resultwhencomparingourleftmostsample with the PCR reference.

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