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Projectlab

Projectlab. PCR cycle. Novablue cells were used. 94 degrees C 30 seconds 64 degrees C 30 seconds 72 degrees C 1 minute After 25 cycles finish with 72 degrees C for 5 minutes. Picture of agarose gel. Our PCR Product Molecular weight marker.

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Projectlab

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  1. Projectlab

  2. PCR cycle Novablue cells wereused • 94 degrees C 30 seconds • 64 degrees C 30 seconds • 72 degrees C 1 minute • After 25 cycles finish with 72 degrees C for 5 minutes

  3. Picture of agarose gel Our PCR Product Molecularweight marker

  4. Our vector after purification, it’sprettyweakbutit’sthere.

  5. Ligation Novablue cells wereused No colonieswereformed after ligation. Therefore a backup culturemade by Lasse wasused for screening and analysis.

  6. Analysis of transformants ReferencePCR fragment from anothergroup Ligatedvector, pretransformation. No vector is visible. Screening of Lasses colonies, you canclearlysee the resultwhencomparingourleftmostsample with the PCR reference.

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