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Projectlab. PCR cycle. Novablue cells were used. 94 degrees C 30 seconds 64 degrees C 30 seconds 72 degrees C 1 minute After 25 cycles finish with 72 degrees C for 5 minutes. Picture of agarose gel. Our PCR Product Molecular weight marker.

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Projectlab

Projectlab


Pcr cycle

PCR cycle

Novablue cells wereused

  • 94 degrees C 30 seconds

  • 64 degrees C 30 seconds

  • 72 degrees C 1 minute

  • After 25 cycles finish with 72 degrees C for 5 minutes


Picture of agarose gel

Picture of agarose gel

Our PCR Product

Molecularweight marker


Projectlab

Our vector after purification, it’sprettyweakbutit’sthere.


Ligation

Ligation

Novablue cells wereused

No colonieswereformed after ligation. Therefore a backup culturemade by Lasse wasused for screening and analysis.


Analysis of transformants

Analysis of transformants

ReferencePCR fragment from anothergroup

Ligatedvector, pretransformation. No vector is visible.

Screening of Lasses colonies, you canclearlysee the resultwhencomparingourleftmostsample with the PCR reference.


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