Hypercholesterolemia
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Hypercholesterolemia PowerPoint PPT Presentation


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Hypercholesterolemia. Pick up sample Get new tip Set to 30 m l Push plunger to 1 st stop Lower tip to bottom of tube Release plunger. Drop off sample Lower tip into well Push plunger to 2 nd stop Raise tip out of well Release plunger Discard tip. Load tray into chamber

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Hypercholesterolemia

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Hypercholesterolemia

Hypercholesterolemia


Hypercholesterolemia

Pick up sample

Get new tip

Set to 30 ml

Push plunger to 1st stop

Lower tip to bottom of tube

Release plunger

Drop off sample

Lower tip into well

Push plunger to 2nd stop

Raise tip out of well

Release plunger

Discard tip

Load tray into chamber

  • Wells to negative end

  • Fill buffer to a level above the gel

  • Double stack

A

B

C

D

E

F


Hypercholesterolemia

Hypercholesterolemia

  • How do our cells use cholesterol?

  • Why does the liver send out cholesterol in LDLs (initially as VLDLs) if HDLs just bring them back?

  • What is the problem with having too much LDL?

  • What method(s) do we have for combatting a high LDL/HDL ratio?

  • A mutated allele for a certain gene leads to FH. For what does the normal allele code?


Hypercholesterolemia1

Hypercholesterolemia

  • What are the genotypes of Patients 1, 2, and 3?

  • Explain two possible reasons for the band locations on gels…

    1st:4 and 5 as compared to 1-3 and 6.

    5th:7-12 as compared to 1-3.

  • Explain how the following changes in procedure would affect band locations:

    • Doubling the agar powder when making gels.

    • Mixing up the connections to the power supply.


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