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1 Database Files of Japanese and Asian Insects

Japanese Bee Fauna, Collections and DNA Barcoding Project Osamu TADAUCHI Entomological Laboratory, Faculty of Agriculture, Kyushu University, JAPAN. 1 Database Files of Japanese and Asian Insects. We have constructed Entomology database KONCHU since 1983, which is a general

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1 Database Files of Japanese and Asian Insects

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  1. Japanese Bee Fauna, Collections and DNA Barcoding ProjectOsamu TADAUCHIEntomological Laboratory, Faculty of Agriculture, Kyushu University, JAPAN

  2. 1 Database Files of Japanese and Asian Insects • We have constructed Entomology database KONCHU since 1983, which is a general database name including various files on insects in Asia and the Pacific area • About 165,000 records are opened via the Internet • One of the files, MOKUROKU is based on “A Check List of the Japanese Insects”

  3. Home page of an Entomology Database KONCHU

  4. Various files in the KONCHU database MOKUROKU KONCHUR TOBOKIBACHI DJI ELKUType

  5. HANABACHI DB based on the Japanese bees

  6. Result of a search: Andrena mikado

  7. Image of various parts Enlarged images are available Female head Male head Mesoscutum

  8. Database File TAB Based on the Tropical Asian Bees

  9. Example of a record: Xylocopa latipes

  10. 2 Bee Fauna in Japan 1 Bee fauna in Japan has been well investigated and revised except for Sphecodes The total number of Japanese bees is 420 spp. based on our MOKUROKU file at present Colletidae: 31spp. Colletes: 6 spp., Hylaeus: 25 spp. Halictidae: 120 spp. Halictus: 4 spp., Lasioglossum 59 spp., Sphecodes: 51 spp. Others: Nomia, Pseudapis, Lipotriches Sphecodes needs revisional work and will decrease the number, Lasioglossum increase it in near future

  11. 2 Bee Fauna in Japan 2 Andrenidae: 87 spp. Andrena: 86 spp., Panurginus: 1 sp. Melittidae: 5 spp. Melitta, Macropis, Dasypoda Megachilidae: 55 spp. Megachile: 34 spp., Osmia: 7 spp., Coelioxys: 5 spp., Hereiades: 4 spp., etc Apidae: 142 spp. Nomada: 52 spp., Bombus: 22 spp., Ceratina: 10 spp., Xylocopa: 6 spp., Amegilla: 5 spp., Tetralonia: 5 spp., etc

  12. 2 Bee Fauna in Japan 3 We are preparing a book on “The Bees of Japan” including all the Japanese species with various images, one page for one species

  13. 3 Bee taxonomists and association in Japan • O. Tadauchi: Andrenidae • Dr. S. Ikudome: Colletidae • Dr. R. Murao: Halictidae (Lasioglossum) • Dr. K. Mitai: parasitic bees (Nomada etc) • Mr. H. Nagase: Megachilidae ------------------------------------------------------- Dr. Y. Hirashima, Dr. Y. Maeta, Dr. M. Ito We have an association of bees in Japan (Jap. Melit. Soc.) and have publish a newsletter once a year. Among 70 members, about 20 persons interest in collecting throughout in Japan

  14. 4 Bee Collections in Japan 1 • Kyushu University, Fukuoka (100,000 indivs.) preserved most types of bees in Japan Japanese bee coll. (60,000 indivs., 20-30y. old) Central Asian coll. (25,000 indivs., 5y. old) Chinese and Korean coll. (10,000 indivs., 10y. old) Other coll. (5,000 indvs., Bangladesh, Iran, Indonesian, Thai and others, 1-15y. old) We are constructing specimen database of bees preserved in our lab. (nearly half finished), which presented to GBIF.

  15. 4 Bee Collections in Japan 3 2 Hyogo Pref. Museum, Hyogo Pref. Types of Tsuneki, normal Col. of Sakagami (20-50y. old) 3 Shimane University, Matsue Japanese coll., 10-40y.old 4 Hokkaido University, Sapporo Japanese coll., 20-70y. old 5 Private Collection: Mr. Haneda, Fukui Pref. Japanese coll., 20-50y. old, large 6 Private Collection: Dr. Munakata, Hokkaido Japanese coll., 20-50y. old, large 7. Others: Toyama City Museum, Toyama, Ibaragi Pref. Museum, Mito, etc

  16. 5 DNA Barcoding Project and Facilities • From April, 2008 we started to collect fresh material for DNA barcoding. At present we preserve about 50 spp., 400 individuals in 99% ethanol. We will ask Japanese bee colleague to collect fresh bee material from this year

  17. 5 DNA Barcoding Project and Facilities • Two graduate students and two undergraduate students in our lab. have started to study DNA barcoding of bees and Cicadellidae (Hemiptera), respectively • We used a universal primer HCO2198 as preliminary work and could get good PCR material • Other laboratory in the same faculty of Kyushu University decided to help us as facilities

  18. Preliminary test of a Japanese Andrena used by universal primer 800bp

  19. 6 Linkage with JBOLI • JBOLI (organized by Prof. Ito of Tokyo University) asked our laboratory to preserve voucher specimen collection of Japanese insects • A new species was described with designation as the first voucher specimen in the Japanese insects (Curculionidae, Coleoptera) preserved in Kyushu University (Yoshitak et al., 2008, Zootaxa)

  20. 7 Construction of a voucher specimen database in Japan • We will construct voucher specimen database of Japanese insects

  21. 8 Funds • I have three funds related to construction of database of Japanese insects from JSPS and JST now (about 930,000 US$ from 2003 to 2008) • From the time being I will use the funds to construct a database file of DNA barcoding as well as the other database files • Associate Prof. of my laboratory succeeded in getting a JSPS fund for DNA barcoding of Cicadellidae (Hemiptera) from 2008 to 2010 • I will apply to JSPS and other national funds such as Ministry of Agriculture for DNA barcoding of useful insects in the next year

  22. 9 Comments of Bees of Other Areas in Asia 1 • We made various surveys and accumulated bee specimens in our laboratory from Korea, China, Southeast Asia, Central Asia, Iran and Papua New Guinea. Central Asian collection is most representative based on five surveys by my project in Kazakhstan and Kyrgyzstan. • I have now foreign students from Iran, Thailand and Egypt who are studying bees. Egyptian student started to study DNA barcoding using Japanese Lasioglossum, Iranian studies Iranian Andrena and Thai studies biodiversity of Thai bees. I will instruct them about DNA barcoding and ask to continue it after coming back from Japan.

  23. 9 Comments of Bees of Other Areas in Asia 2 • One of my students from China, Dr. Xu, got a job in Chinese Agricultural University in Beijing and is interested in DNA barcoding of bees. He will ready to join this project. • We are now constructing TAB and TABRdatabase based on tropical Asian bees, which will contribute to the study of bees in this area. • Prof. Yata of Kyushu University, who is a specialist of butterflies, got JSPS funds and succeeded in constructing network of entomologists and institutes in southeast Asia. I think we can use this network for accumulating and collecting bee specimens.

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