DNA Extraction Lab. Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study. DNA Extraction Process involves four parts :. Put the cells into a solution. Use enzymes to hydrolyze the cell wall.
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DNA Extraction Lab
Step by step procedure that weakens the outer boundaries of a cell and lyses it to release the DNA for future study.
The above is the bacteria used in the DNA extraction lab. Note its Spherical appearance and the color after gram staining. The gram stain specifically stains the cell wall. According to this picture, is it gram positive or negative?
Check out the Bacteria Power Point to find out about Gram Positive and Negative Staining…..
By adding these solutions to a test tube of bacteria, a lysate is made that contains DNA.
Contents of the lysate after shaking, rotating and inverting the tube:
The lysate is then placed in a hot water bath set at 60-65 degrees Celsius. Why? Enzymes denature at high temperatures. Nucleases are enzymes that digest DNA so the hot water bath destroys the damaging nucleases in the lysate.
In this picture, the student used 95% ethanol to cause the DNA to precipitate out of the lysate. DNA is soluble in the lysate but insoluble in ethanol. By drawing the lysate up into the alcohol layer, the DNA comes in contact with the ethanol, thus, becoming visible to the naked eye.
6. DNA should attach to the spooling rod at the aqueous-ethanol interface.