Biocompatibility of sapphire and borosilicon glass using combined mri and histology
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Biocompatibility of Sapphire and Borosilicon Glass Using Combined MRI and Histology. Kaushik S Parthasarathy. Aim of the Research. We developed a Protocol to investigate the short and long term neuro compatibility of novel and traditional Biomaterials that could be used in Neural Prosthesis

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Biocompatibility of sapphire and borosilicon glass using combined mri and histology l.jpg

Biocompatibility of Sapphire and Borosilicon Glass Using Combined MRI and Histology

Kaushik S Parthasarathy


Aim of the research l.jpg
Aim of the Research Combined MRI and Histology

  • We developed a Protocol to investigate the short and long term neuro compatibility of novel and traditional Biomaterials that could be used in Neural Prosthesis

  • Wafers of Sapphire and BSG (2.5mm dia. x 0.250mm thick) were implanted unilaterally onto the surface of the parietal cortex using aseptic techniques in rats and imaged using MRI (4.7T Animal Magnet)


Experiments l.jpg
Experiments…. Combined MRI and Histology

  • The animals were allowed to survive for 28 days

  • Sham – operated and mechanically – lesioned animals served as controls

  • MRI

    • Pre and Post implantation (10, 28 days) MRI was performed using Spin Echo and Gradient Echo Sequence, on sham, +ve control and implanted animal

    • Assessment of Inflammation, Edema and Tissue interference with the implant was done using MRI

  • Histology

    • Tissue underlying the implant was stained for degenerating axons and neurons using Nissl, Silver, GFAP for reactive astrocytes stains


Results l.jpg
Results Combined MRI and Histology

Sham

10th Day 28th Day

10th Day 28th Day


Histology l.jpg
Histology Combined MRI and Histology

Nissl Stain


Sapphire implant l.jpg
Sapphire Implant Combined MRI and Histology

10th Day 28th Day


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Histology Combined MRI and Histology

Nissl Stain GFAP Silver


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Future Directions Combined MRI and Histology

  • Surface modification is done in order to enhance the biocompatibility of the materials

    “GOAL”

  • The overall goal of the project is to provide comprehensive evaluations of materials and neural implants in the most appropriate (in the retina and the brain where the structures will be implanted for treatment or diagnoses) setting.


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Acknowledgements Combined MRI and Histology

  • Michigan Life Science Corridor (MLSC) for funding the Research

  • E.Mark Haacke

  • Dr.Pat McAllister

  • Dr.G.Auner

  • Dr.Norman Cheng

  • Yimin Shen

  • Jie Li

  • Kelley Barbant


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