the effects of nitric oxide on the development of alzheimer s disease
Download
Skip this Video
Download Presentation
The Effects Of Nitric Oxide On The Development Of Alzheimer\'s Disease

Loading in 2 Seconds...

play fullscreen
1 / 17

The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease - PowerPoint PPT Presentation


  • 95 Views
  • Uploaded on

The Effects Of Nitric Oxide On The Development Of Alzheimer\'s Disease. Caroline Hsiao. Need. http://www.alz.org/advocacy/2006program/images/charts/5.gif. Need. http://msnbcmedia1.msn.com/i/msnbc/Components/Art/HEALTH/070319/AP_ALZHEIMERS.gif. Plaques. Tangles in Neuron. Plaques.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease' - daisy


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
the effects of nitric oxide on the development of alzheimer s disease

The Effects Of Nitric Oxide On The Development Of Alzheimer\'s Disease

Caroline Hsiao

slide2
Need

http://www.alz.org/advocacy/2006program/images/charts/5.gif

slide3
Need

http://msnbcmedia1.msn.com/i/msnbc/Components/Art/HEALTH/070319/AP_ALZHEIMERS.gif

plaques
Plaques

Tangles in Neuron

Plaques

slide5

Alzheimer’s Disease

http://pampee.20gig.com/wp-content/uploads/2009/04/alzheimers-disease.jpg

http://www.alzheimer.ca/english/disease/whatisit-intro.htm

slide6

Alzheimer’s Disease

http://www.topnews.in/health/files/alzheimers-brain.jpg

slide7

Nitric Oxide As A Free Radical

http://www.inchem.org/documents/jecfa/jecmono/40abcj11.gif

http://www.inchem.org/documents/jecfa/jecmono/40abcj11.gif

oxidative stress
Oxidative Stress
  • Cigarette smoke is used as an example shown here

http://healthy-lifestyle.most-effective-solution.com/wp-content/uploads/2007/10/oxidative-stress.jpg

age dependent neurodegeneration and alzheimer amyloid plaque formation in transgenic drosophila
Age-Dependent Neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila

Greeve, and Kretzschmar,

slide10
Dissecting the pathological effects of human A40 and A42 in Drosophila: A potential model for Alzheimer’s disease

By: Iijima and Liu

purpose
Purpose

The purpose of this experiment was to find if nitric oxide have any effect on the development of Alzheimer\'s disease.

Alternate: Nitric Oxide will cause an increase in the development of Alzheimer\'s symptoms in drosophila for all groups tested.

Null Hypothesis: there will be no change in any of these groups at all.

experimental design
Experimental Design

Protocols Used:

GrittaTettweiler, Mathieu Miron, Mark Jenkins, 2005

ELIZABETH J. HARRY, KIT POGLIANO, AND RICHARD LOSICK 1995

slide14

The Effects of Nicotine and Nitric Oxide on the development of Amyloid beta plaques in wild type and APP drosophila

Wild Type – no nitric oxide n = 20

APP Drosophila–no nitric oxide n= 20

  • APP Drosophila –nitric oxide for 1 minute n= 20 (6 days old)
  • APP Drosophila –nitric oxide for 5minute n= 20 (6 days old)

Wild Type–nitric oxide for 1 minute n= 20

Wild Type–nitric oxide for 1 minute n= 20

Wild Type-nitric oxide n= 20 (6 days old)

5 trials will be run, each for approximately 12-13 days per trial.

After all slides have been made they will be observed using the fluorescent microscope and the images will be taken using a digital camera and then using Photoshop the total amount of amyloid beta plaques will be measured.

Using SPSS statistical analysis will be done using an ANOVA followed by a Scheffe Posthoc test with p< .05

do ability
Do Ability
  • All materials are orderable from school approved companies
  • Drosophila can be kept alive with relative ease in school lab
  • School lab already has a microscope necessary for observation
bibliography
Bibliography
  • Crag A. Michelli, William P Esler and W. Taylor Kimberley, Y-Secretase/presenilin inhibitors for Alzheimer’s disease phenocopyNotch mutations in Drosophila, FASEB, 3/09/2003, volume 17, pgs 79-81
  • Crowther DC, Kinghorn KJ, Miranda E, Page R, Curry JA, et al. (2005) IntraneuronalAb, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer’s disease. Neuroscience 132: 123–135.
  • Harry EJ, Pogliano K, Losick R (1995) Use of immunofluorescence to visualize cellspecific gene expression during sporulation in Bacillus subtilis. J Bacteriol
  • 177, volume 12, pgs 3386-93.
  • GrittaTettweiler, Mathieu Miron, Mark Jenkins, Nahum Sonenberg, and Paul F. Lasko, Starvation and oxidative stress resistance in Drosophila are mediated through the eIF4E-binding protein, d4E-BP, Genes Dev, August 15 2005, volume 19, pgs 1840–1843.
  • IsabellGreeve, Doris Kretzschmar, Jakob-Andreas Tscha¨pe, AnikaBeyn, Claire Brellinger, Michaela Schweizer, Roger M. Nitsch, and Rita Reifegerste1, Age-Dependent neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila, The Journal of Neuroscience, April 21, 2004, volume 16, pgs 3899 –3906
  • Koichi Iijima, Hsin-Ping Liu, Ann-Shyn Chiang, Stephen A. Hearn, Mary Konsolaki, Yi ZhongDissecting the pathological effects of human Aβ40 and Aβ42 in Drosophila: A potential model for Alzheimer\'s disease, PNAS, October 31, 2003, volume 101, pgs 6623–6628
  • MUHAMMAD ARSHAD, MARIE PAULSSON, and PETR DEJMEK, Rheology of Buildup, of Acid Casein Gels Breakdown, and Rebodying, March 3 1993, J Dairy Sci volume 76, pgs 331-3316
  • P. Mpountoukas, A. Vantarakis, E. Sivridis and T. Lialiaria, Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives, January 30 2008, Food and Chemical Toxicology, volume 46, pgs 2390-2393
  • Pogliano K, Harry E, Losick R (1995) Visualization of the subcellular location of
  • sporulation proteins in Bacillus subtilis using immunofluorescence microscopy. Mol
  • Microbiol, volume 18, pgs 459-70
  • Yihong Ye and Mark E. Fortini Apoptotic Activities of Wild-type and Alzheimer’s Disease-related Mutant Presenilins in Drosophila melanogaster, The Journal of Cell Biology, September 20 1999, Volume 146, pgs 1351–1364
ad