Bartonella. Prepared by: Ohood R. Sarsour. Introduction. Bartonella species (formerly known as Rochalimaea) Linked to a number of emerging zoonotic diseases B.quintana, B.bacilliformis, B.henselae
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Ohood R. Sarsour
Western blot and cross-adsorption results in a patient
with Bartonella quintana endocarditis. A) Nonadsorbed. B)
Adsorbed with B. quintana. C) Adsorbed with B. henselae. Lane 1,
B. quintana; lane 2, B. henselae; lane 3, B. elizabethae; lane 4, B.
vinsonii subsp. Berkhoffi; lane 5, B. vinsonii subsp. Arupensis.
Before adsorption (A), antibodies are detected against all species
(1, 2, 3, 4, and 5). After adsorption with B. quintana antigen (B), all
antibodies disappear. After adsorption with B. henselae antigen
(C), antibodies against B. quintana (1) persist. This reaction shows
B. quintana infection.
. Immunohistochemical demonstration of Bartonella sp
Laser confocal microscopy showing the intraerythrocytic
location of Bartonella quintana.
Gram negative aerobic, pleomorphic, flagellated, motile, coccobacillary, 2-3 m large and 0,2 - 0,5 m wide and facultative intracellular bacterium.
For its isolation, special cultures are required containing complemental soy agar, proteases, peptones, some essential amino acids and blood. The optimum growing temperature is 19-29 ºC.
Phlebotomine sand flies
Phlebotomine sand flies
Values in porcentaje
The diagnosis in the acute phase can be done using the thin blood film with Giemsa stain.
It is possible to observe the bacillus inside the red blood cells.
Immunologic technics: Sonicated immunoblot
Lane A: Positive control pool
Lane Band C:Bartonella bacilliformis-positive serum taken from a patient in acute phase
Lane D: Negative control pool
A B C D
M: DNA ladder (100 bp).
1:B. bacilliformis DNA from culture extracted by thermal lysis (100°C, 10 min.) using 16S 23S primers (positive control).
2: Whole blood extraction from an acute phase patient, using 16S 23S primers.
3: Whole blood extraction from an acute phase patient, using primers for Citrate Synthetase gene.
4: B. bacilliformis DNA from a culture extraction using primers for Citrate Synthetase gene.
M 1 2 3 4
Miliary lesions with overwhelming infection