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Evaluation of positive Predictive Value of Enzyme-linked Immunosorbent Assay and Stool Culture used for the Detection of Campylobacter spp from stool sample. Taj Muhammed 1 , Nazneen Tabassum 1 , Shazia Perween 1 , Asif Fida, 1 Furqan Kabir, Shahida Mumtaz Qureshi 2 ,

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Background

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  1. Evaluation of positive Predictive Value of Enzyme-linked Immunosorbent Assay and Stool Culture used for the Detection of Campylobacter spp from stool sample Taj Muhammed1 , Nazneen Tabassum 1, Shazia Perween 1, Asif Fida, 1 Furqan Kabir, Shahida Mumtaz Qureshi2, Dr. Anita Zaidi2 and Dr. Zulfiqar Bhutta2 Dept Of Paediatrics and Child Health, Division Women and Child Health Background Campylobacter is a gram negative, microaerophilic bacterium and is one of the most common bacterial causes of diarrheal illness in the world. Campylobacter organisms can be found everywhere and are commonly found in the intestinal tracts of cats, dogs, poultry, cattle, swine, rodents, monkeys, wild birds, and humans. Enzyme-linked Immunosorbent Assay (ELISA) and stool culture techniques used to detect Campylobacter Spp. Positive predictive value (PPV) is the proportion of subjects with positive test results who are correctly diagnosed. It is a critical measure of the performance of a diagnostic method, as it reflects the probability that a positive test reflects the underlying condition being tested for. Its value does however depend on the prevalence of the outcome of interest, which may be unknown for a particular target population. Methodology 50 stool samples were tested both for ELISA and stool culture out of which 13 were positive by both ELISA and Stool Culture 08 were positive by ELISA not by Culture. PPV were applied to evaluate the techniques. Objective To evaluate the positive predictive value of Enzyme-linked Immunosorbent Assay (ELISA) and stool culture used for the detection of Campylobacter Spp. from stool culture. Conclusions Results shown Stool Culture correctly identifies 62% of cases which were true positive for Campylobacter Spp., while the Sensitivity of culture technique found to be 93%. Key Words: PPV, ELISA, Stool Culture and Campylobacter spp. Result The PPV is found to be 62%, whereas sensitivity & specificity is found to be 93% and 78% respectively. REFERENCE: MAL-ED Project Birth Cohort Studies Black,R.E.,etal. caulfield,L.E.,etal. Victora,C.G.,etal. Campbell,D.I,M.Elia and P.G.Lunn 1183 - Ped/ERC-09  9.73

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