Corn genetics chi square goodness of fit test
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Corn Genetics & Chi- Square Goodness of Fit Test. Pedigrees and probability analysis to follow. Outline. Highlight some key points in syllabus Corn color and collection of data (phenotypes) Chi-Square Analysis. Corn genetics Lab. Application of Chi-Square Analysis.

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Corn genetics chi square goodness of fit test l.jpg

Corn Genetics &Chi- Square Goodness of Fit Test

Pedigrees and probability analysis to follow.


Outline l.jpg
Outline

  • Highlight some key points in syllabus

  • Corn color and collection of data (phenotypes)

  • Chi-Square Analysis



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Application of Chi-Square Analysis

  • The color pattern in corn is due to multiple genes

  • Are variations in number of observed phenotypes due to chance alone?


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Objective

  • Complete corn worksheet

  • Set up Punnett Squares using the genotypes given

  • Compare expected data to observed data

  • Perform Chi Square Analysis



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Corn Kernel Color

  • Color of aleurone masks endosperm color

  • Only with colorless aleurone can endosperm color be determined

  • Determined by several genes

  • Refer to genotypes in lab manual!


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Gene interaction can occur:Epistasis is a form of gene interaction in which one gene masks the phenotypic expression of another.


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Today there are: 3 crosses of corn

F1 X F1

Multiple alleles are tested for expression in F2 generation


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Note color differences

  • Cross C

  • Cross D (darker color is purple)

  • Cross F (call purple, may look red!)


Cross c l.jpg

F1 phenotype

F1 X F1 genotypes

Yellow

YYC’Rr X YYC’Rr

Cross C


Cross d l.jpg

F1 phenotype

F1 X F1 genotypes

Purple

yyPpRr X yyPpRr

Cross D


Cross f l.jpg

F1 phenotype

F1 X F1 genotypes

Purple

yyCcRr X yyCcRr

Cross F


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Chi Square Statistical “Goodness of Fit Test”

  • Why do we use it?

  • .

  • To find out determine if probability are due to chance alone.

An example of how to calculate a Chi Square



Genetic information regarding the corn color patterns are provided in your lab manual l.jpg
Genetic information regarding the corn color patterns are provided in your lab manual.

CORN WORKSHEET IS DUE 3/5


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