Structure of Macromolecules

1. Structure of Macromolecules …by small and simple things are great things brought to pass.

2. Macromolecules Molecular weight exceeding 1000 Macromolecules are polymers constructed by the formation of covalent bonds between smaller molecules called monomers

3. Condensation and Hydrolysis Monomers are joined by condensation reactions, which release a molecule of water for each bond formed. Hydrolysis reactions use water to break polymers into monomers.

4. Molecular organization in the cell is a hierarchy

6. 4 BASIC BIOLOGICAL MACROMOLECULES • Proteins • Carbohydrates • Lipids • Nucleic acids

7. Proteins Proteins are polymers composed of hundreds or even thousands of amino acids linked in series by peptide bonds. Basic building block is the amino acid The functions of proteins include support, protection, catalysis, transport, defense, regulation, and movement.

8. What is amino acid? • Amino means? • Acid?

9. There are 20 amino acids commonly found in proteins • peptide linkages form by condensation reactions between the carboxyl and amino groups of adjacent amino acids.

10. Amino Acids Cysteine Tyrosine Phenylalanine Glutamic acid Threonine Proline Valine Histidine Glutamine Serine • Alanine • Arginine • Lysine • Glycine • Asparagine • Methionine • Isoleucine • Aspartic acid • Tryptophan • Leucine

11. Proteins: Structure • Primary Structure • Secondary Structure • Tertiary Structure • Quaternary Structure

12. Proteins: Structure Primary Structure: the sequence of amino acids bonded by peptide linkages (Diversity 20n) (covalent bonding)

13. Even a slight change in the amino acid sequence can cause the protein to malfunction For example, A single amino acid substitution in hemoglobin causes sickle cell disease

14. Secondary Structure Results from hydrogen bonding between the oxygen of one amino acid and the hydrogen of another(non covalent interactions) α helices and β pleated sheets (maintained by hydrogen bonds between atoms of the amino acid residues)

15. The alpha helix is a coiled secondary structure due to a hydrogen bond every fourth amino acid

16. The beta pleated sheet is formed by hydrogen bonds between parallel parts of the protein

17. A single polypeptide may have portions with both types of secondary structure

18. Tertiary Structure Generated by bending and folding of the polypeptide chain 1) Covalent disulfide bridges, 2)Hydrophobic interactions 3) van der Waals forces 4) Ionic bonds

19. Quaternary structureresults from interactions among separate polypeptide chains.

20. Proteins: Denaturation A loss of three-dimensional structure sufficient to cause loss of function is called denaturation. Proteins are denatured by heat, alterations in pH, or certain chemicals lose their tertiary and secondary structure as well as their biological function. Renaturation is not often possible.

21. Carbohydrates Carbohydrates are polyhydroxy aldehydes or ketones, or substances that yield such compounds on hydrolysis They act as source of energy that can be transported They also have structural roles

22. classification • Monosaccharides • Disaccharides • Oligosaccharides (3-20) • Polysaccharides

23. Monosaccharides The monosaccharides are also called simple sugars and have the formula (CH2O)n. Monosaccharides cannot be broken down into smaller sugars

24. Ring form of sugars • There are two forms of the ring structure (α-glucose and β-glucose), which differ only in the placement of the —H and —OH attached to carbon 1. • The α and β forms (called anomers) interconvert and exist in equilibrium when dissolved in water.

25. Glycosidic linkages Covalently link monosaccharides into larger units such as disaccharides, oligosaccharides, and polysaccharides Digestible

26. Polysaccharides The polysaccharides are sugar polymers containing more than 20 or so monosaccharide units; some have hundreds or thousands of units. Some polysaccharides, such as cellulose, are linear chains; others, such as glycogen, are branched. For example Cellulose: Glucose polysaccharide β Linkages Starch: Glucose polysaccharide α LinkagesGlycogen: Glucose polysaccharide Branched

27. Polysaccharides

28. Modified Carbohydrates

29. Lipids Lipids are a class of biological molecules defined by low solubility in water and high solubility in nonpolar solvents. As molecules that are largely hydrocarbon in nature, lipids represent highly reduced forms of carbon and, upon oxidation in metabolism, yield large amounts of energy. Lipids are thus the molecules of choice for metabolic energy storage.

30. Water insoluble due to nonpolar covalent bonds • Hydrophobic molecules aggregate together (by hydrophobic and Van der Waals force) • Store energy as triglycerides • Phospholipids form cell membranes • Carotenoids help plants capture light energy • Steroids are hormones and vitamins • Animal fat is thermal insulator • Insulation of nerves • Water repellant for skin, fur and feathers

31. Fats and oils are triglycerides, composed of three fatty acids covalently bonded to a glycerol molecule by ester linkages. Triglycerides

32. Lipids: Saturated and unsaturated Saturated fatty acids have a hydrocarbon chain with no double bonds. The hydrocarbon chains of unsaturated fatty acids have one or more double bonds that bend the chain, preventing close packing.

33. Phospholipids Phospholipids contain fatty acids bound to glycerol by ester linkages. In phospholipids, however, any one of several phosphate-containing compounds replaces one of the fatty acids. The phosphate functional group has a negative electric charge, so this portion of the molecule is hydrophilic, attracting polar water molecules. But the two fatty acids are hydrophobic, so they tend to aggregate away from water.

34. A variety of polar groups are esterified to the phosphoric acid moiety of the molecule. The phosphate, together with such esterified entities, is referred to as a “head” group

35. Lipids in Aqueous Cell Environment The interactions of the hydrophobic tails and hydrophilic heads of phospholipids generate a phospholipid bilayer that is two molecules thick. The head groups are directed outward, where they interact with the surrounding water. The tails are packed together in the interior of the bilayer.

36. Lipids in Vitamins and hormones Vitamins are small molecules that are not synthesized by the body, but are necessary for its normal functioning. There are four lipid soluble vitamins- vitamin A,D,E and K. Many hormones are also lipid in nature e.g cortisol.

37. Nucleic Acids • Nucleic acids are linear polymers of nucleotides. • Nucleotides have three characteristic components: (1) a nitrogenous (nitrogen-containing) base, (2) a pentose, and (3) a phosphate. • Nucleotide without the phosphate group is called a nucleoside.

38. Nitrogenous bases • The nitrogenous bases are derivatives of two parent compounds, pyrimidine and purine. • Both DNA and RNA contain two major purine bases, adenine(A) and guanine(G), and two major pyrimidines. • In both DNA and RNA one of the pyrimidines is cytosine(C), but the second major pyrimidine is not the same in both: it is thymine(T) in DNA and uracil (U) in RNA

39. Purines are double ring bases e.g. adenine & guanine Pyrimidines are single ring bases e.g. cytosine, thymine, uracil

40. Nucleoside • Nucleosides are compounds formed when a base is linked to a sugar. Nucleosides are composed of : (1) a nitrogenous base, (2) a pentose • Examples : Adenosine Guanosine, Cytidine, Thymidine, Uridine

41. Nucleotides • Nucleotides have three characteristic components: (1) a nitrogenous base, (2) a pentose, and (3) a phosphate. • Examples: Adenylate (AMP) Guanylate (GMP) Cytidylate (CMP) Thymidylate (TMP) Uridylate (UMP)

42. Nucleic acids • Nucleic acids are linear polymers of nucleotides • Examples: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)

43. structure • In both RNA and DNA, the backbone of the macromolecule consists of alternating pentose sugars and phosphates (sugar—phosphate—sugar—phosphate). The bases are attached to the sugars and project from the chain.

44. The nucleotides are joined by phosphodiester linkages between the sugar of one nucleotide and the phosphate of the next .The phosphate groups link carbon 3 in one pentose sugar to carbon 5 in the adjacent sugar.

45. Most RNA molecules consist of only one polynucleotide chain. DNA, however, is usually double-stranded; it has two polynucleotide strands held together by hydrogen bonding between their nitrogenous bases.

46. The two strands of DNA run in opposite directions. This antiparallel orientation is necessary for the strands to fit together in three-dimensional space. • The uniqueness of a nucleic acid resides in its nucleotide sequence

47. Only four nitrogenous bases—and thus only four nucleotides—are found in DNA: adenine (A), cytosine (C), guanine (G), and thymine (T). In double-stranded DNA, adenine and thymine always pair (A-T), and cytosine and guanine always pair (C-G). (complementary base pairing. )

48. Differences between DNA RNA single stranded Contains ribose Contains uracil Participates in the expression of genetic information stored in the DNA Three types rRNA, mRNA, tRNA • Double stranded • Contains deoxyribose • Contains thymine • Stores genetic information

49. Nucleic Acids

50. DNA Double Helix has Uniform Width Information in Sequence not Shape