Recording Your Research (Lab Books, Research Diaries and Electronic Information). Dr Richy Hetherington, Dr Catherine Exley and Dr Dan Swan. Outline. General Guidance Recording Lab Work Research Diaries – Recording research outside of the lab environment Electronic recording
Dr Richy Hetherington, Dr Catherine Exley and Dr Dan Swan
All should be
Always describe thoroughly a new procedure
“Solution B was made by adding 500µl of water to 1.02mg of substance B, in 1.5ml microfuge tube, the solution was vortexed for 30 minutes to ensure Substance B was dissolved.
Would be better than
“A 2mg/ml of Solution B was made from substance B”
Once methods are established it is fine to refer back to them
“Samples B1-B16 were tested using method A (see 14th June 2007 RH/B1/ p14)”
Obviously note any deviations, intentional or otherwise
“Samples B1-B16 were tested using method A (see 14th June 2007 RH/B1/p14). Deviating from the method Samples B13-B16 were centrifuged twice at 14.2g for 5 minutes in stage 2, all other samples were centrifuged once as per method A.”
“Samples B1-B16 were tested using method A (see 14th June 2007 RH/B1/ p14). Deviating from the method all samples were centrifuged at 14.2g for 10 minutes at stage 2, to ensure a compact pellet formed.
Equipment used list
Reagents used list
Make note of anything anecdotal or unusual.
“The cells appeared to be more irregular in shape, than in previous experiments”
“The interview room was very warm; some of the subjects seemed slightly unconformable”
“Centrifuging for 10 minutes at stage 2 had no perceivable effect on the compactness of the pellet. When repeating increasing the rotor speed to produce 15g may help compact the pellet”
“Excessive centrifuging of samples B1-16 reduced enzyme activity, solids in stage 2 must be collected by filtering”
(at least fortnightly)
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