BIOL 4014 Microbiology   Fall 2008
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BIOL 4014 Microbiology Fall 2008 Mon Wed, Lecture 8 am 9:40 am, LSW 444; Lab 9:00-10:50 am or 2:00-3:50 pm, LS - PowerPoint PPT Presentation


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BIOL 4014 Microbiology Fall 2008 Mon & Wed, Lecture 8 am – 9:40 am, LSW 444; Lab 9:00-10:50 am or 2:00-3:50 pm, LSW 546 Instructor: Dr. David F. Gilmore Office: LSE 418 Phone 972-3263 Email: [email protected] Web: http://www.clt.astate.edu/dgilmore Office hours: TBA

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BIOL 4014 Microbiology Fall 2008

Mon & Wed, Lecture 8 am – 9:40 am, LSW 444; Lab 9:00-10:50 am or 2:00-3:50 pm, LSW 546Instructor: Dr. David F. Gilmore

Office: LSE 418 Phone 972-3263

Email: [email protected] Web: http://www.clt.astate.edu/dgilmore Office hours: TBA

other times by appt. or just drop by.Lab text: Microbiology Laboratory Theory and Application 2nd ed by Leboffe and Pierce. Required


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  • Integrated Lab/Lecture course

  • Match up of lecture topics and Lab exercises

  • Additional time for discussion of lecture material, lecture exams.

  • Avoid “is this lab or lecture?” thinking.

    • Except for Lab Final, exams cover lecture and lab.

  • Powerpoint lectures

  • Each slide numbered for your reference

  • Listen to ME, take smart notes

  • Powerpoints will be posted before class, but attendance is expected!

  • No textbook, but online reading assignments!

  • Stuff

  • Schedules may change, more likely in lab

  • Alteration in points for Lab assignments

  • Don’t be a stranger.

  • Please no cell phone use or ringing in class.


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  • Grading

  • Four regular exams, 100 points each

  • Final (fifth) Exam, 100 points.

  • Attendance in lecture: 50 points.

  • Lab assignments totaling 450 points, typically

    • 3 or 4 Lab Reports

    • One Lab Final

    • One Unknown identification

  • Total 1000 points

Student email for official commuication

my web page: study guides, lab report directions, etc.

Cheating

Full syllabus is posted online


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Microbiology: Definitions

  • Microbiology: study of living things too small to be seen w/o a microscope.

    • What’s life?

      • Highly organized, self replicating, self-adjusting, capable of evolving, can obtain energy

      • Made of cells, comes from pre-existing cells

    • Are all microbes that small?

      • Epulopiscium and Thiomargarita: visibly large bacteria


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Classification of Microbes

  • Three domains

    • Eubacteria: prokaryotic cell structure

    • Archaebacteria: prokaryotes, but different

    • Eukaryotes: 4 kingdoms

      • Plants, animals, fungi, and protists.

  • What are microbiologists interested in?

    • Eubacteria and archaebacteria for sure.

    • Eukaryotes like fungi and protists

    • Animals (parasitic worms) but not really plants.

    • What’s missing?

      • VIRUSES!


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Divisions of Microbiology

  • By critter type:

    • Bacteriology, virology, mycology

    • Parasitology (includes protozoa, fungi, worms)

  • Other divisions:

    • Pathogenic microbiology, Immunology, molecular biology, microbial ecology

    • Applied microbiology: water treatment, natural products, food microbiology, environmental microbiology


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History: Ancient knowledge

  • Recognition of Immunity:

    • Variolation and protection from infection

      • Intentional contact with minor form of smallpox

        • Protected against Variola major

    • Edward Jenner and cowpox (1796)

      • Milkmaids catch cowpox, seem to be immune to smallpox.

  • Contagion: disease can be spread by contact.

    • Exclusion of lepers; burning of plague victims

    • Catapulting of disease victims into castles during siege


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Satire on Jenner and vaccination

encarta.msn.com/.../ Vaccination_with_Cowpox.html


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History continued

  • Microbiology as a biological science

    • Robert Hooke, 1665, discovery of cells

    • Antony van Leeuwenhoek, father of microbiology

      • Dutch amateur lens grinder

      • First person to see microbes, late 1600s

    • Mid 1800s, microbes taken more seriously and studied using the scientific method

micro.magnet.fsu.edu/.../ introduction.html


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History: the Golden Age

  • From about 1850 to start of 20th century

  • Read about significant individuals of this era:

    • Louis Pasteur

    • Robert Koch

    • Ignaz Semmelweis

    • Joseph Lister

    • Paul Ehrlich

  • Major concepts

    • Fermentation, other chemical processes due to life

    • Germ theory of disease: sicknesses caused by microbes

    • Aseptic technique; avoid infection, contamination


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20th Century Microbiology

  • Molecular biology

    • Use of microbes as model systems for study

    • Study of DNA, proteins synthesis

    • Tools and processes for recombinant DNA

  • Applied microbiology

    • Food industry

    • Water and sewage treatment

    • Bioremediation

  • Medicine

    • Emerging diseases; antibiotic resistance


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    • Why Study Microbes?

    • Major impact on health

      • Responsible for disease in humans, animals, plants

    • Major impact on environment

      • Major decomposers

      • Nutrient cycling, elemental cycling

    • Microbes are talented

      • Live under extreme conditions

      • Protect against disease

      • Eat oil, toxic waste (bioremediation)

      • Make plastic

      • Spoil food, make food

      • Use light, produce light


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