An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay. Seiji Watanabe1,*, Atsushi Tanaka2, Shunsaku Fujii3, Hideki Mizunuma3, Atsushi Fukui3, Rie Fukuhara3, Rika Nakamura3,
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Seiji Watanabe1,*, Atsushi Tanaka2, Shunsaku Fujii3,
Hideki Mizunuma3, Atsushi Fukui3, Rie Fukuhara3, Rika Nakamura3,
Kenichi Yamada3, Izumi Tanaka2, Shoichiro Awata2, and
Human Reproduction, Vol.0, No.0 pp. 1–9, 2011
In the large vacuole group, normal-shaped sperm exhibiting large vacuoles with a diameter of ›1.5 mm were selected from the sperm drop and then injected into mouse oocytes in a drop of HEPES-HTF medium
Thanks for attention only spontaneous DNA damage, but also ‘potential’ DNA damage caused by denaturation. Using sperm fixed with paraformaldehyde