The effect of caffeine on the survival and development of mealworms
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The Effect of Caffeine on the Survival and Development of Mealworms. By: Anna Gillin. Question. How does caffeine effect the growth, survival, and development of mealworms?. Research - Caffeine. Caffeine (C 8 H 10 N 4 O 2 ) is a drug that stimulates the nervous system

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Presentation Transcript

Question
Question Mealworms

  • How does caffeine effect the growth, survival, and development of mealworms?


Research caffeine
Research - Caffeine Mealworms

  • Caffeine (C8H10N4O2) is a drug that stimulates the nervous system

  • It gives consumers an increase in energy and can elevate mood

  • Caffeine is added to drinks and foods

  • Side effects vary according to consumer


Research mealworms
Research - Mealworms Mealworms

  • Mealworms are the larva stage of the Darkling Beatle

  • Mealworms are part of the Tenebrio Genus and the T. molitor species

  • The larval stage of mealworms can last from 90 up to 114 days

  • As mealworms grow, they shed old shells and develop new ones.


Hypothesis
Hypothesis Mealworms

  • The group of mealworms exposed to high levels of caffeine will lose more mass and have a higher death rate than the groups exposed to lower levels of caffeine.


Materials
Materials Mealworms

  • Wheat Bran

  • Corn meal

  • Shredded Newspaper

  • Mortar and Pestle

  • Digital Balance

  • 500 milligrams caffeine

  • 100 milligrams caffeine

  • 135 mealworm larvae

  • Square plastic containers

  • Metal screens

  • Distilled Water


Procedure
Procedure Mealworms

  • The mealworms were divided into 3 groups:

    • Group A (no caffeine)

    • Group B (low caffeine)

    • Group C (high caffeine)

  • 15 mealworms were put into each box

  • The following solutions were made:

    • Group A: 100 mL of distilled water

    • Group B: 100 mgs ( ½ caffeine pill) in 100 mL of distilled water

    • Group C: 500 mgs (2 ½ caffeine pill) in 100 mL of distilled water


Procedure1
Procedure Mealworms

  • 50 mL of the solutions were mixed with 50 grams of wheat bran and 50 grams of cornmeal

  • The mass and survivors were measured after a week and new bedding, solutions, and food mixtures were made

  • The length of the experiment lasted 4 weeks


Variables
Variables Mealworms

  • Independent Variable- the amount of caffeine given to each group of mealworms

  • Dependent Variable- the effects that caffeine had on each group of mealworms and the number of mealworms still living

  • Control- the group of mealworms (Group C) that was not exposed to caffeine and only had food moistened by water

  • Constants- the type of container, metal screen, temperature, food, bedding in the container, and amount of mealworms in each group


Data Mealworms


Data Mealworms


Data Mealworms


Data Mealworms


Data Mealworms

Graph 1

Graph 2


Data Mealworms

ttest of the Number Dead

ttest of the Percent Change in Mass

ttest of the Percent Change in Mass/Organism


Conclusion
Conclusion Mealworms

  • The hypothesis stated that the mealworms exposed to higher levels of caffeine would lose more mass and have a higher death rate

  • The hypothesis was partially supported because caffeine had a negative effect on mealworms

  • It was rejected that varying levels of caffeine have different effects on mealworms


Improvements
Improvements Mealworms

  • The temperature should remain constant

  • A different source of food should be used

  • The environment should remain consistent


Further studies
Further Studies Mealworms

  • Different organisms could be tested with caffeine

  • The same experiment could be performed for the mealworms in their adult form as beetles

  • A wider range of caffeine levels could be used to see if different amounts of caffeine have the same effects


Works cited
Works Cited Mealworms

  • Mealworm Store. (n.d.). Mealworm Facts and Information. In Mealworm Facts and Information. Retrieved 2007, from Mealworm Store website: http://mealwormstore.com/‌mealworm_info.php

  • Smith Life Science. (2009, September 29). Mealworm Diagram. In Mealworms. Retrieved October 5, 2010, from Smith Life Science website: http://www.smithlifescience.com/‌MLMealworms.htm

  • University of Arizona. (1997). Darkling Beetle/‌ Mealworm Information . Retrieved October 21, 2010, from Center for Insect Science Education Outreach website: http://insected.arizona.edu/‌mealinfo.htm

  • Majithia, N. (2008). Caffeine: Understanding the World’s Most Popular Psycoactive Drug. Retrieved October 7, 2010, from Journal of Young Investigators website: http://www.jyi.org/‌features/‌ft.php?id=1327

  • Gavin, M. L., & Black, J. D. (2008). Caffeine. Retrieved October 19, 2010, from The Nemours Foundation website: http://kidshealth.org/‌teen/‌drug_alcohol/‌drugs/‌caffeine.html#

  • Kovacs, B., & Stoppler, M. C. (n.d.). Caffeine. In Caffeine [Caffeine, Health Effects, Statistics]. Retrieved October 5, 2010, from MedicineNet website: http://www.medicinenet.com/‌caffeine/‌article.htm

  • Lieberman, H. R., Tharion, W. J., Shukitt-Hale, B., Speckman, K. L., & Tulley, R. (n.d.). Biomedical and Life Sciences. In Effects of Caffeine on Sleep loss, and Stress on Cognitive Performance and Mood During U.S. Navy SEAL Training [Caffeine, Effects ]. Retrieved October 5, 2010, from SpringerLink website: http://www.springerlink.com/‌content/‌2bjn0v6dg712phw5/


Thank you
Thank You Mealworms

  • Please feel free to ask any questions regarding my experiment.


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