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Cancer Cell Detection in Tissue using AFM - Journal Club

This journal club presentation discusses the mechanical characterization of cancer cells in different tissue sections using Atomic Force Microscopy (AFM). The study examines the elastic properties of breast and prostate cancer cells, as well as different tissue samples of uterine corpus, breast cancer, and vulvar cancer.

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Cancer Cell Detection in Tissue using AFM - Journal Club

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  1. Journal Club Andrew Lau Mechanics of Advanced Materials Healthcare Engineering/Centre of Biological Engineering 30th September 2015 Wolfson School of Mechanical and Manufacturing Engineering

  2. Cancer cell detection in tissue sections using AFM Archives of Biochemistry and Biophysics (518) 2012, pp 151 - 156 Małgorzata Lekka a, Dorota Gil b, Katarzyna Pogoda a, Joanna Dulin´ ska-Litewka b, Robert Jach c, Justyna Gostek a, Olesya Klymenko a, Szymon Prauzner-Bechcicki a, Zbigniew Stachura a, Joanna Wiltowska-Zuber a, Krzysztof Okon d, Piotr Laidler b a The Henryk Niewodniczan´ski Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland b Chair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, 31-034 Kraków, Poland c Chair of Gynaecology and Obsterics, Department of Gynaecological Oncology, Jagiellonian University Medical College, Kopernika 23, 31-501 Kraków, Poland d Chair of Pathomorphology, Jagiellonian University Medical College, Grzegórzecka 16, 31-531 Kraków, Poland http://dx.doi.org/10.1016/j.abb.2011.12.013 Wolfson School of Mechanical and Manufacturing Engineering

  3. Archives of Biochemistry and Biophysics Impact Factor 3.017 5-year Impact Factor 2.980 Source Normalized Impact factor per paper (SNIP) 0.875 SCImago Journal Rank (SJR) 1.188 Wolfson School of Mechanical and Manufacturing Engineering

  4. Background • PhD Title ‘Mechanical Characterisation of Soft Biomaterials and Tissues’ • Supervisors: Prof Vadim Silberschmidt • Dr Yang Liu • Dr Simin Li • April 2014 • 2nd Year PGR • Study microscopic structure of biological materials • Appearance of normal/abnormal cells • Operation of AFM to measure mechanical properties • Determine moduli of normal/abnormal cells Wolfson School of Mechanical and Manufacturing Engineering

  5. INTRODUCTION • Cancer progression characterise by disruption of the cytoskeleton and molecular alterations affecting mechanical properties. • Determination of cell stiffness in relation to cancer progression. • Elastic properties of breast and prostate cancer were determined in the study Wolfson School of Mechanical and Manufacturing Engineering

  6. MATERIALS • CELL LINE • non-tumorigenic prostate cells (PZHPV-7) • prostatic adenocarcinoma (PC-3) • metastatic prostate adenocarcinoma (Du 145) • metastatic prostate carcinoma (LNCaP) • normal mammary (A184A1) • breast cancer (T47D) • breast adenocarcinoma (MCF-7) • TISSUE SAMPLE • Nonneoplstic endometrium Well differentiated endometroid carcinoma • Nonneoplastic breast cancer Infiltrating ductal carcinoma • Nonneoplastic vulvar tissue Vulvar cancer Wolfson School of Mechanical and Manufacturing Engineering

  7. METHOD • System • Atomic Force Microscopy Model Xe120 (Park Systems) • Force Spectroscopy Mode in liquid conditions • Scan area of 10 – 12 μm • Data Analysis • Hertz contact model • where F is the force applied and δ is the indentation depth. • 4 sided pyramid (35° MLCT type C cantilever) • α is the open angle and μcell is the Poisson ratio (0.5) F=λδβ Wolfson School of Mechanical and Manufacturing Engineering

  8. Wolfson School of Mechanical and Manufacturing Engineering

  9. Wolfson School of Mechanical and Manufacturing Engineering

  10. Results • Cell Line Samples Wolfson School of Mechanical and Manufacturing Engineering

  11. Results • Cell Line Samples Wolfson School of Mechanical and Manufacturing Engineering

  12. Results • Tissue Samples – Uterine corpus Wolfson School of Mechanical and Manufacturing Engineering

  13. Results • Tissue Samples – Breast cancer Wolfson School of Mechanical and Manufacturing Engineering

  14. Results • Tissue Samples – Vulvar cancer Wolfson School of Mechanical and Manufacturing Engineering

  15. Conclusion Wolfson School of Mechanical and Manufacturing Engineering

  16. Conclusion Wolfson School of Mechanical and Manufacturing Engineering

  17. Conclusion • For all cell types, the normal cells are characterised by a larger Young’s modulus value as compared to their cancerous counterpart. • Normal cells have a lower ability to deform compared to cells from late stages of cancer progression • Tissue sections studied also show same pattern Wolfson School of Mechanical and Manufacturing Engineering

  18. Remarks • Indentation depth • from 200 nm to 1500 nm, show decrease in Young’s modulus value for both cancerous and normal breast cells respectively • Ramp velocity • from 0.5 μm/s to 10.0 μm/s, show increase in change in Young’s modulus of 48% and 157% for cancerous and normal breast cells (400nm) Wolfson School of Mechanical and Manufacturing Engineering

  19. Thank You for Listening Wolfson School of Mechanical and Manufacturing Engineering

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