Antibody-Functionalized Carbon Nanotubes in Cancer Therapy * Kyung Kim**, Kristina Tran**, and Dr. Miguel Bagajewicz University of Oklahoma—Chemical Engineering. * This work was done as part of the Capstone chemical engineering class at the University of Oklahoma
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Antibody-Functionalized Carbon Nanotubes in Cancer Therapy*
Kyung Kim**, Kristina Tran**, and Dr. Miguel Bagajewicz
University of Oklahoma—Chemical Engineering
* This work was done as part of the Capstone chemical engineering class at the University of Oklahoma
** Capstone undergraduate students
Mathematical Modeling for Tumor Targeting
A mathematical model is used to determine appropriate timing of an existing cancer treatment based on attaching monoclonal antibodies (mAb) to single-walled carbon nanotubes (SWNT) for breast cancer cells. The mathematical model predicts the initial dosage for intravenous injection and near-infrared (NIR) radiation emission intensity and timing as a function of the tumor volume.
Temperature Gradient of the Tumor Cell at
Various Irradiation Times
Plasma & Lymph Flow Diagram
t = 1 min
t = 2 min
t = 3 min
The two-pore model shows how mAbs travel through the organs and tissues.
It also explains the diffusion of mAbs into the interstitium through the blood vessel wall.
Two Subcompartments Model
Immunoglobulin IgG is dispersed to the vascular and interstitial spaces through plasma flow. Their half-life is determined by the FcRn receptor in endothelial cells. This model describes the catabolic process of IgG through its internalization into the organ and tissues.
(on breast cancer cells)
Single-walled carbon nanotube
We have successfully modeled
Based on the temperature gradient of the tumor during irradiation, damage to the surrounding normal cells will be minimal. It is not desirable to have the tumor cells reach temperatures above 80°C due to bubbling of the tumor and subsequent damage to surrounding normal cells. Therefore, an irradiation time of three minutes is sufficient for eradication of tumor cells.
Due to their small size, nanosystems have been shown to overcome biological barriers, such as the blood-brain barrier, which makes them attractive for cancer therapy.
kint : Rate constant of internalization
kcat: Rate constant for lysosomal degradation
krec: Rate constant for recycling of Fc-Rn bound
Cm: Concentration of catabolites
CLorg: Catabolite clearance rate
Jv : Fluid flux
αL, αS : Fraction of bulk fluid
Functionalizing SWNTs with mAbs
Concentration of mAbs in the Body
The functionalization of SWNTs with mAbs serves to deliver SWNTs to the cancer site for phototherapy.
Begin NIR irradiation
We would like to thank the following for their guidance and help in our project: Chiranth Srinivasan, Dr. Daniel Resasco, Dr. Lance Lobban, Dr. Liang Zhang, Dr. Barbara Safiejko-Mroczka, and Kevin Bagnall.
.Shao, N., Lu, S., Wickstrom, E., and Panchapakesan, B., Integrated molecular targeting of IGF1T and HER2
surface receptors and destruction of breast cancer cells using single wall carbon nanotubes, Nanotechnology (2007), 18:1-9.
2. Davda, J. P., Jain, M., Batra, S. K., Gwilt, P. R., Robinson, D. H., A physiologically based pharmacokinetic (PBPK) model to characterize and perdict the disposition of monoclonal antibody CC49 and its single chain Fv constructs, International Immunopharmacology (2008) 8:401-413.
3. Meyskens, F., Thomson, S., Moon, T., Quantitation of the Number of Cells within Tumor Colonies in Semisolid Medium and Their Growth as Oblate Spheroids, Cancer Research (1984), 271-277.
4. G. Z. Ferl, A. M. Wu, J. J. Distefano III, A predictive model of therapeutic monoclonal antibody dynamics and regulation by the newnatal Fc Receptor (FcRn), Annals of Biomedical Engineering (2005) 33( 11): 1640-1652.
Shao, N., Lu, S., et al., Integrated molecular targeting of IGF1T and HER2 surface receptors and destruction of breast cancer cells using single wall carbon nanotubes, Nanotechnology (2007), 18:1-9.
University of Maine, Department of Chemistry, <http://chemistry.umeche.maine.edu/CHY431/Antibody.jpg>