Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics. R. R. R. Go R. A 600 reading show the growth for 3 consecutive readings. 4 ml. Shake. Subculture E. coli o/n. 40 ml LB in R and C. 4 ml. C. C. C. Go C.
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Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics.
A600 reading show the growth for 3 consecutive readings
Subculture E. coli o/n
40 ml LB in R and C
Centrifuge at 4, 000rpm for 15min at 37°C. Wash the cells twice with 20ml fresh LB media prewarmed to 37°C (see note 4). Resuspend the final pellet (see note 5) to 40ml in a clean 250ml shake flask.
3 consecutive readings
Take reading every 10 min
Continue incubation at 37°C, with shaking, and read A600 every 15min for a further 60-90min.
Prepared by Angelia Teo Jan 09
Plot graph and discuss