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Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics. PowerPoint PPT Presentation


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Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics. R. R. R. Go R. A 600 reading show the growth for 3 consecutive readings. 4 ml. Shake. Subculture E. coli o/n. 40 ml LB in R and C. 4 ml. C. C. C. Go C.

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Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics.

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Lab 1 the bacteriostatic and bactericidal action of antibiotics

Lab 1: The Bacteriostatic and Bactericidal Action of Antibiotics.

R

R

R

Go R

A600 reading show the growth for 3 consecutive readings

4 ml

Shake

Subculture E. coli o/n

40 ml LB in R and C

4 ml

C

C

C

Go C

Centrifuge at 4, 000rpm for 15min at 37°C. Wash the cells twice with 20ml fresh LB media prewarmed to 37°C (see note 4). Resuspend the final pellet (see note 5) to 40ml in a clean 250ml shake flask.

Shake

1ml ethanol

(2mg chloramphenicol)

3 consecutive readings

Take reading every 10 min

Shake

Continue incubation at 37°C, with shaking, and read A600 every 15min for a further 60-90min.

1ml methanol

(1mg rifampicin)

Prepared by Angelia Teo Jan 09

Plot graph and discuss


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