1 / 12

Broad-band Decoupling

Broad-band Decoupling. Decoupled 1-D NMR Pulse Sequence. Basic 1-D NMR Pulse Sequence. 1-D NMR of a protein. 2-D NOESY of a protein. 1H/1H-COSY NMR. Mass Spectrometry. Chapters 11 and 20. Example Mass Spectrum. Dalton – amu Molecular ion peak

clara
Download Presentation

Broad-band Decoupling

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Broad-band Decoupling Decoupled 1-D NMR Pulse Sequence Basic 1-D NMR Pulse Sequence

  2. 1-D NMR of a protein 2-D NOESY of a protein

  3. 1H/1H-COSY NMR

  4. Mass Spectrometry Chapters 11 and 20

  5. Example Mass Spectrum

  6. Dalton – amu Molecular ion peak – m/z corresponding to MW of singly charged molecule Fragment peak – m/z less than MW of singly charged molecule Base peak – m/z peak that is most intense

  7. Hard ion source – leaves excess energy in ion – extensive fragmentation Soft ion source – leaves less energy in ion – reduced fragmentation

  8. Hard vs Soft Ionization

More Related