The Role of Sphingolipids in Lipid Raft Function in Paramecium tetraurelia. Tyler Picariello 12/7/10. Outline. Background Model Organism Cilia and Lipid Rafts Methods Expected Results. Paramecium Background.
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The Role of Sphingolipids in Lipid Raft Function in Paramecium tetraurelia
Pantel, Haddon; Undergraduate Honors Thesis, 2007
Image courtesy of Megan Valentine
Adapted from http://www.ncbi.nlm.nih.gov/books/NBK26892/
Disruption of sphingolipids, a key component of ciliary lipid rafts, through the depletion of the serine palmitoyltransferase (SPT) gene message will result in disruption of ciliary lipid raft formation. This will in turn disrupt Folate chemoattraction and ciliary calcium channel function.
To study the effect of serine palmitoyltransferase mRNA depletion on lipid raft formation in Paramecium. SPT mRNA depletion will be achieved through the RNAi feeding method.
I. The effects of SPT mRNA depletion on lipid raft organization will be analyzed by sucrose density gradient centrifugation.
II. Study the effects of SPT mRNA depletion on Folate chemoattraction using T-Maze assays
III. Study the effects of SPT mRNA depletion on ciliary calcium channel function using backward swimming assays.
RNAi by feeding
Adapted from Haddon Pantel and Mellissa Donovan