Pharos university Faculty of Allied Medical SCIENCE Clinical Laboratory Instrumentation (MELI-201 ). Dr. Tarek El Sewedy. Lecture 7. Cytometry & Flowcytometers AND Auto analyzers. Intended Learning Outcomes. Students will learn : Principles and instruments used in of Cytometry techniques
Dr. Tarek El Sewedy
Cytometry & Flowcytometers
Students will learn :
These slides allow the counting of cells in a small volume and extrapolating the result to determine the total number.
Some flow cytometers have the capability of separating cells from a mixture of cells based on characteristics determined by the scientist. This is achieved adding a device called cell sorter to the Flowcytometer. The cell sorter places cells into separate containers based on size or other characteristics.
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.
Always consider any laboratory specimen as potentially infectious and handle it carefully; wear protective gloves.
Once filled, containers should be autoclaved or soaked in disinfectant before burning or burying in a deep pit.