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Production des particules lentivirales

Production des particules lentivirales. Lentiviral genome. LTR. gag. pol. env. LTR. Packaging plasmid. Prom. gag. pol. Envelope plasmid. Integrating plasmid. Prom. env. LTR. Prom. Transgene. LTR. 293T cell. Lentiviral particles.

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Production des particules lentivirales

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  1. Production des particules lentivirales Lentiviral genome LTR gag pol env LTR Packaging plasmid Prom gag pol Envelope plasmid Integrating plasmid Prom env LTR Prom Transgene LTR 293T cell Lentiviral particles

  2. Lentiviral transduction of zygotes and ES cells Lentiviral particles Injection of the lentiviral particles in the perivitelline space of the fertilized egg Incubation of the fertilized egg minus the zona pellucida With the lentiviral particles Incubation of ES cells with the lentiviral particles Rate of embryo survival +++ Rate of transgenesis +++ Transgene expression +++

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