بسم الله الرحمن الرحيم
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بسم الله الرحمن الرحيم. Oxidative Decarboxylation and Krebs Cycle. By. Amr S. Moustafa, M.D.; Ph.D. Clinical Biochemistry Unit, Pathology Dept. College of Medicine, King Saud University. ALT. Alanine. PLP. αKG. Glutamate. Fates of Pyruvate. Oxidative Decarboxylation of Pyruvate.

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Oxidative decarboxylation and krebs cycle
Oxidative Decarboxylation and Krebs Cycle

By

Amr S. Moustafa, M.D.; Ph.D.

Clinical Biochemistry Unit, Pathology Dept.

College of Medicine, King Saud University


Fates of pyruvate

ALT

Alanine

PLP

αKG

Glutamate

Fates of Pyruvate



Pdh complex covalent regulation
PDH Complex: Covalent Regulation

Insulin

+

PDH

Insulin

Glucagon

-

PDH

P

+

Pi

H2O

Protein Phosphatase

Pyruvate dehydrogenase

complex (active)

Pyruvate dehydrogenase

complex (inactive)

Protein Kinase

ATP

ADP

+

Glucagon


Tricarboxylic acid cycle krebs cycle
Tricarboxylic Acid Cycle: Krebs Cycle

  • Final common pathway for oxidation

  • Exclusively in mitochondria

  • Major source for ATP

  • Mainly catabolic with some anabolic features

  • Synthetic reactions (anabolic features):

  • Glucose from amino acids

  • Nonessential amino acids

  • Fatty acids

  • Heme




Krebs cycle reactions 2
Krebs Cycle Reactions (2)

Succinate Thiokinase

Substrate-Level

Phosphorylation


Phospho glycerate kinase

Glycolysis

(Cytosol)

Phospho-glycerate Kinase

2

2

Substrate-

Level

Phosphorylation

2

2

2

Substrate-

Level

Phosphorylation

Pyruvate Kinase

2





Net atp production by complete glucose oxidation
Net ATP Production byComplete Glucose Oxidation

Aerobic glycolysis: 8 ATP

Oxidative decarboxylation: 2 X 3 = 6 ATP

Krebs cycle: 2 X 12 = 24 ATP

Net: 38 ATP


Take home message
Take Home Message

  • Pyruvate is oxidativelydecarboxylated by PDH to acetyl CoA inside the mitochondria

  • Krebs cycle:

    • Final common pathway for the oxidation of carbohydrates, fatty acids and amino acids

    • occurs in the mitochondria

    • Aerobic

    • Mainly catabolic, with some anabolic reactions

  • The complete oxidation of one glucose molecule results in a net production of 38 ATP molecules



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