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Resolución ejercicio de purificación por cromatografía

Resolución ejercicio de purificación por cromatografía. Marzo, 2011. Resinas (3): Carboxi metil (CM)- sefadex G25, Dietil amino etil (DEAE)-celulosa, Sefadex-G25 Amortiguadores: 1 M Acetato de potasio pH 6.0; 0.1M Acetato de potasio pH 6.0 2M HCl.

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Resolución ejercicio de purificación por cromatografía

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  1. Resolución ejercicio de purificación por cromatografía Marzo, 2011

  2. Resinas (3): • Carboximetil (CM)-sefadex G25, • Dietilaminoetil (DEAE)-celulosa, • Sefadex-G25 Amortiguadores: • 1 M Acetato de potasio pH 6.0; • 0.1M Acetato de potasio pH 6.0 • 2M HCl. • Y la disponibilidad de diluirlas si así lo desean.

  3. Hay muchas resinas disponibles en el mercado como:

  4. Columnas de intercambio iónico sugeridas: • Matrix agarose, dextran, acrylamide, same as for gel filtration exclusion limit. • Charged groups I. Positively charged groups anion exchangers: -DEAE (di-ethylaminoethyl) weak anion -QAE (quaternary aminoethyl) strong anion II. Negatively charged groups cation exchangers: -CM (carboxymethyl) weak cation -SP (sulfopropyl) strong cation

  5. Purificación mediante cromatografías sucesivas, este es un ejemplo pero podrían haber sido varias las respuestas Eluyente DEAE 1M HCl CM 1M KAc

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