Would glucose or lactose be more beneficial for the growth of P. fluorescens ? - PowerPoint PPT Presentation

Would glucose or lactose be more beneficial for the growth of p fluorescens
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Would glucose or lactose be more beneficial for the growth of P. fluorescens ?. Hyun Suk Park Anh Trinh Carissa Best. Glucose. The Critter. Pseudomonas fluorescens Gram negative, rod-shaped Thrives in environments of 25 degrees Celsius

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Would glucose or lactose be more beneficial for the growth of P. fluorescens ?

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Would glucose or lactose be more beneficial for the growth of p fluorescens

Would glucose or lactose be more beneficial for the growth of P. fluorescens?

Hyun Suk Park

Anh Trinh

Carissa Best

Glucose


The critter

The Critter

Pseudomonas fluorescens

  • Gram negative, rod-shaped

  • Thrives in environments of 25 degrees Celsius

  • Able to grow on a variety of media including lactose


Why we picked lactose

Why we picked lactose?

To examine the effects on growth rate in comparison to glucose.

20% concentration of Glucose

OD

Time(min)


Glucose vs lactose

Glucose vs. Lactose

P. Fluorescens growth on lactose or media enriched culture

OD

-Glucose(3%)

-Lactose(3%)

Time(min)


Lactose concentrations

Lactose concentrations

Further explore an effective amount of lactose at the lowest concentration possible to steady the growth rate

OD

Time(min)


Conclusion

Conclusion

  • Lactose effectively controlled the overall growth of P. fluorescens in comparison to glucose within a certain range

  • At very low concentrations of lactose a more accurate transfer method is needed to have significant measures of the effects on overall growth


Next time

Next Time

  • Possible further experiments with additional time

  • Better dilution method/procedure

  • Lactose solution settling and preparation


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