1 / 18

Pheromones in interactions of hyphae

Pheromones in interactions of hyphae. Fusion of mating compatible strains. Clamp cell fusion is B mating type controlled. Fusions occur between colonies of the same mating type and fusions occur between hyphae within a colony. Homing reactions, peg formations

chase
Download Presentation

Pheromones in interactions of hyphae

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Pheromones in interactions of hyphae

  2. Fusion of mating compatible strains Clamp cell fusion is B mating type controlled

  3. Fusions occur between colonies of the same mating type and fusions occur between hyphae within a colony

  4. Homing reactions, peg formations Fusions: tip to tip, tip to peg, peg to peg

  5. H P Antagonism by hyphal interference: Phlebiopsis against Heterobasidion Oidial homing: Hyphae of own or different species are attracted to oidia of Coprinopsis, fuse with them and might be killed

  6. Hyphal fusion events in a colony of Neurospora crassa. Colony stained with FM4-64. (From Hickey et al., 2002)

  7. Model for hyphal fusion in Neurospora crassa

  8. The B mating type locus of higher basidiomycetes encodes pheromones and pheromone receptors phB1.2-1 phB1.1-1 rcB1-1 B1 B2 phB1.2-2 phB1.1-2 rcB1-2 O´Shea et al. 1998, Halsall et al. 2000

  9. The B43 mating type locus in C. cinerea spans up to 66 kb on chromosome X rcB5 rcB6 25 kb phB3.2 phB3.1 rcB3 msf2 msf1 phB3.3 8 kb 8 kb phB2.1 phB2.2 phB1.1 rcB1 phB1.2 phB2.3 phB2.4 rcB4 rcB2 chromosome V: rcB47 T.Y. James, U. Kües

  10. The B locus in Laccaria spans 17.5 kb with 5 receptor genes: Blast with Ste3 of Cryptococcus 9 other receptor genes on other scaffolds (3 incomplete) 3 pheromone genes

  11. Potential B pheromones in Laccaria(scaffold 56) MDEFTTLFS.....DSLS..EHA...C...NGNDSIPSDI...PLD.EDSQYRPAI.WCTIS MDSFTSLESLCESVDFITFQEDFSSSC...SSHQPISSDL...PIDQERYNAGNTGAFCVIS MDSFSTF.......DLSTF.EPTSSSLPISSAHQTEDDDILRQLVD.EDTKLGYAG.YCVIA

  12. 34 (+1 nonfunctional) pheromones from other scaffolds MDAF.TFYITS.VDAIPIDEKTEVESLPTDEES.GGSGNNAYCVIA MDAF.TFYITSGVDAIPINEKTDIESAPTDEDN.GGSGNNAYCVIA MDAF.TFFVD..IETTFTHHQTQGDSIPTNGES.GGSGNNAYCVIA MDAF.TLFFN..VDTVFTNEQTQGDSIPTSEES.GGSGNNAYCVIA MDAF.AELVDYLSTPTVTTVGGDVASVPTNEED.RGTGNNAYCVVA MDAF.TLFAN..IDTVFTNEQTQGDTIPTNEES.GGSGNNAYCVIA MDTF.TTYSAYSADVEDNT.KFT...IPVDKAS.GRRGNHAYCIIS MDTF.TTYSAYSADVEDNT.KFT...IPVDKES.GGRGNHAYCIIS MDSF.TTYFSLAAKAEDI..SSQ...APVDEES.GGSGNNAYCVIA MDAF.TTYSTYSADAEDNT.IFT...IPVD.EESGGRGNNAYCIFS MDAF.TTYSTYSADAEDNT.IFT...IPVD.EESGGRGNNAYCIFS MDAF.TTYSAYSADAEDNT.IFT...IPID.EESGRRGNNAYCLNS MDSF.TSLFTVVASA..VSGPTE...IPTNADD.GGSGNNAYCVVA MDSF.TTLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLA...TFFASISGSSD...IPTNADD.GGSGNNAYCVVA MDSF.GA.....IEL.TGTG.SH...IPTDQEHQ.GPGQWAYCVVA MDSF..NVAE..ITT.TP.G.SD...IPINQEHQGGAGQYAYCVIA MDAF........ITT.TV.DVDN...IPVCKEQPGGSGNDVYCTIA MDSF.ATLSS..IFF.SVSGPTD...IPTNADD.GGSGNNAYCVVA MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLS...IFFAAVSGPSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLT...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.TTICN..........TSD...IPTNSED.GGSGNNAYCVIA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSFFTTISN..........TSN...IPTNSDD.GGSGNNAYCVVA MDSFFTTISN..........TSD...IPTNSDD.GGSGNNAYCVVA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVVA MDSF.STIST..........STD...IPTN.YEGGGSGNNAYCVIA MNSF.STISTS.........TGS...IPTN.YEGGGSGNNAYCVIA MDSF.STISTS.........TDS...IPTN.YEGGGSGNNAYCVIA MDAFATYSPYSADAEDDTHFTIPVDEESG*RGNHAYCIVS

  13. 34 (+1 nonfunctional) pheromones from other scaffolds MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLS...IFFAAVSGPSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLT...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSF.ATLA...IFFASVSGSSD...IPTNADD.GGSGNNAYCVVA MDSF.TTICN..........TSD...IPTNSED.GGSGNNAYCVIA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVIA MDSFFTTISN..........TSN...IPTNSDD.GGSGNNAYCVVA MDSFFTTISN..........TSD...IPTNSDD.GGSGNNAYCVVA MDSF.TTISN..........TSN...IPTNSDD.GGSGNNAYCVVA MDSF.STIST..........STD...IPTN.YEGGGSGNNAYCVIA MNSF.STISTS.........TGS...IPTN.YEGGGSGNNAYCVIA MDSF.STISTS.........TDS...IPTN.YEGGGSGNNAYCVIA MDAF.ATYSPYSADAEDDT.HF...TIPVDEES.G*RGNHAYCIVS

  14. MDSFTT..ITS.FTNKPSVEVPSDLETGGGSGNGAYCVIA MDSFAT..ITS.FTNKPSVEVPSDLETGGGSGNGAYCVIA MDSFTT..IAS.FTNKPTVEVPSDLETGGGSGNGAYCVIA MDSFTT..IAS.FTNQPSVEVPSDLETGGGSGNGAYCVIA MDSF..TAITS.FTNQPSVEVPSDLETGGGSGNGAYCVIA MDSFTT..IAS.FTNQPSVEVPSDLETGGGSGNGAYCVIA MDSFTT..IAS.FTNQPSVEVPSDLETGGGSGNGAYCVIA MDSFTTTAITS.FTNQPSVEVPSDLETGGGSGNGAYCVIA MDSF..TAITSIFTTQPSVEVPSDLETGGGSGNGAYCVIA MDSF..TAIIPVFTTQPSVEVPSDLETGGGSGNGAYCVIA MDSFTT.IVSSIFTTQPSVEVPADNETGGGSGNGAYCVIA MDSFAT.IVSSIFTTQPSVEVPADNETGGGSGNGAYCVIA MDSFT.KFSTDIPTSQ.........ETGGNNGGGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.V..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGGGN MDSFTT.I..NIPTNF.........ETGGG-GNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFTIFSALFTSSSATSGAPATAEAVSTILQVPTDFETGGGSGNGAYCVIA MDSFTIISNVRDVTS.....VPTNEETGGGRGNGAYCVVA MDSFTTLFSNNVSATTS...VPVEEETGGGRGTGAYCTIA MDSFTIFSNNVNEITS....IPTEEEVAGGRGNGAYCIIA MDSFTLFSNNVSATTS....VPAEEETGGGRGTGAYCTIA MDAFATLFSTVFPSTNVDDITNTPTPSVPVEEETGGGRGTGAYCVIA MDAFTTF......DNY.....PIDRETGGGGDPGAYCTIA MDFFTT.IFASQVADLEPVSVPAEEETGGGRGTGAYCTIA MDASITSGN..........IPLDKETGGGAGNGAHCIIA MDSFTTVLSIFTTSQVEDVFVPVEEESGKKGGSGAYCTIA MDSFTIFSALFTSSSATSGAPATAEAVSTILQVPTDFETGGGSGNGAYCVIA MDSFTT.I..NIPTNF.........ETGGGSGNGAYCVIA MDSFQTICFSSEIAAAPNASANVDSSTTGAGVPVVAAAAPVIEIPVDEERNPWAGIWGGKMM.CTIA Consensus Laccaria GGSGNNAYC Coprinopsis GGGSGNGAYC

  15. The surrounding of the B locus 3 pheromone genes

  16. B mating type loci of different species C. cinerea RAB1.1 CCUP8 PERO/cip CCUP7.1 CCUP7.2 STE20/Cla4 RPS19 phB1.2 rcB1 15 kb 31 kb P. djamor B mating type loci of different species PDSTE3.3 AKOR1 AKOR2 PDUPB1 PDUPB2 RPS19 STE20/CLA4 CHODH PDphb1 5 kb 13 kb PDSTE3.1 PDSTE3.2 C. disseminatus CDSTE3.1 CDPHB1 PERO CDUP8 CDRT CDPHB2.1 CDSTE3.3 CDPHB2.2 CDUP9 12 kb CDSTE3.4 CDSTE3.2 ERG26 CDUP7 RAB1.2 RAB1.1 James et al. 2004, T.Y. James, U. Kües

More Related