RBC Manual Count. Introduction. Red blood cells make up almost 45 percent of the blood volume. Their primary function is to carry oxygen from the lungs to every cell in the body.
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In certain conditions, Hayam’s solution may cause clumping of RBCs and Rouleaux formations, while Gower's solution prevents these problems
Whole blood using EDTA or heparin as anticoagulant. Capillary blood may also be used.
(Pipettes) used one of the following:
Thoma pipette (RBCs)
Micropipette –20l is the desired volume.
Improved Neubauer chamber with the cover slips.
Conventional light microscope.
1. Dilution of the blood: