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In certain conditions, Hayam’s solution may cause clumping of RBCs and Rouleaux formations, while Gower's solution prevents these problems
Whole blood using EDTA or heparin as anticoagulant. Capillary blood may also be used.
(Pipettes) used one of the following:
Thoma pipette (RBCs)
Micropipette –20l is the desired volume.
Improved Neubauer chamber with the cover slips.
Conventional light microscope.
1. Dilution of the blood: