Cooperative heavy metal detoxification by p aeruginosa
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Cooperative heavy-metal detoxification by P. aeruginosa. Siobhán O ’ Brien, Adela Luján & Angus Buckling College of Life and Environmental Sciences, University of Exeter Cornwall Campus, UK Email: [email protected] Siderophore production: A model system for studying cooperation. Fe2+.

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Cooperative heavy-metal detoxification by P. aeruginosa

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Cooperative heavy metal detoxification by p aeruginosa

Cooperative heavy-metal detoxification by P. aeruginosa

Siobhán O’Brien, Adela Luján & Angus Buckling

College of Life and Environmental Sciences,University of Exeter Cornwall Campus, UK

Email: [email protected]


Cooperative heavy metal detoxification by p aeruginosa

Microbial communities- Cambridge


Siderophore production a model system for studying cooperation

Siderophore production: A model system for studying cooperation

Fe2+

West SA & Buckling A. 2003 Proc.R.Soc.Lond.B 270:37 – 44.

Griffin AS et al. Nature. 430 :1024 – 1027

West SA et al 2007 Annu. Rev. Ecol. Evol.Syst.38:53 – 77

Braud et al. 2010. Environ Microbiol. 2: 419 – 425

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Our Questions

Part 1

Part 2

Part 3

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

How do we determine if a behaviour is altruistic?

There is a benefit to the recipient

Do cooperators outcompete cheats when grown alone?

The behaviour itself is costly

Do cheats outcompete cooperators in competition?

WT

WT

Ch

Ch

WT

WT

Ch

WT

WT

WT

Ch

Ch

Ch

WT

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Is heavy metal detoxification by siderophores a cooperative trait?

Wt

Ch

1:1

(X12)

6mM CuSO4

Strains:

Wt: PAO1

Ch:PAO1ΔPvdΔPch

Copper Sulfate

Cheat

Cooperator

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Is heavy metal detoxification by siderophores a cooperative trait?

Do cooperators do better than cheats when grown alone in toxic environments? ✔

Do cheats outcompete cooperators in competition in toxic environments? ✔

Cheat relative fitness ~ metal treatment: conditions: F1,89 =207.89, p < 0.001)

O’Brien, Hodgson & Buckling. Social Evolution of toxic Metal Bioremediation in P. aeruginosa. 2014.Proc.R.Soc.Lond.B 281(1787)

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Is heavy metal detoxification by siderophores a cooperative trait?

Experimental evolution (approx. 105 generations)

WT

WT

X12

Copper

Iron

Frequency of cheats

Total siderophore (pyoverdine) production

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Is heavy metal detoxification by siderophores a cooperative trait?

Conclusion:

Copper detoxification is a social trait that actively selects for the evolution of social “cheats”.

***

***

O’Brien, Hodgson & Buckling. Social Evolution of toxic Metal Bioremediation in P. aeruginosa. 2014.Proc.R.Soc.Lond.B 281(1787)

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Our Questions

Part 1

Part 2

Part 3

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

How is cheat fitness affected by the presence of the soil microbial community?

Cheat (species 2)

Cooperator (species 1)

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

How is cheat fitness affected by the presence of the soil microbial community?

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

How is cheat fitness affected by the presence of the soil microbial community?

Monoculture

Co-culture

Cheat relative fitness (w)

Cheat relative fitness (w)

+Cu

+Cu

+Cu

+Cu

-Comm. + Comm. - Comm. + Comm.

-Comm. + Comm. - Comm. + Comm.

(cheat fitness~ copper*community, p=0.03). Error bars represent +/-SE

(cheat fitness~ copper, p=0.01). Error bars represent +/-SE

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

  • Summary

  • Heavy metal detoxification by bacterial siderophores is a cooperative behaviour, that selects for rapid evolution of social ‘cheats’

  • In toxic copper soil, cheat fitness is increased in the presence of the community, but in monoculture conditions only.

  • Cheats cannot ‘invade’ populations of cooperators in soil as they can in synthetic media.

Microbial communities- Cambridge


Thanks for listening

Thanks for listening!

  • Angus Buckling

  • Dave Hodgson

  • Adela Luján

  • Florian Bayer

  • Elze Hesse

  • Eleanor Van Veen

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

How is cheat fitness affected by the presence of the soil microbial community?

Cu2+

Cheat

Cu2+

Cu2+

Cu2+

Cu2+

Cooperator

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cheat (species 2)

Microbial communities- Cambridge


How is cheat fitness affected by the presence of the soil microbial community

How is cheat fitness affected by the presence of the soil microbial community?

Monoculture

Co-culture

Cheat relative fitness (w)

Cheat relative fitness (w)

(cheat fitness~ copper*community, p=0.03). Error bars represent +/-SE

(cheat fitness~ copper, p=0.01). Error bars represent +/-SE


Siderophore production a model system for studying cooperation1

Siderophore production: A model system for studying cooperation

transportation

Fe3+

Fe2+

Fe2+

Mineral/protein

Fe3+

Fe3+

siderophore producer

West SA, Buckling A. 2003 Cooperation, virulence and siderophore production in bacterial parasites. Proc.R.Soc.Lond.B 270:37 – 44.

Griffin AS, West SA, Buckling A. 2004 Cooperation and competition in pathogenic bacteria. Nature. 430 :1024 – 1027

West SA, Diggle SP, Buckling A, Gardner A, Griffin AS. 2007 The social lives of microbes. Annu. Rev. Ecol.Evol.Syst.38:53 – 77.

Microbial communities- Cambridge


Cooperative heavy metal detoxification by p aeruginosa

Siderophores bind other heavy metals too…

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

Cu2+

siderophore producer

…but don’t take them into the cell

Braud A, Geoffroy V, Hoegy F, Mislin GLA, Schalk IJ. 2010 Presence of the siderophores pyoverdine and pyochelin in the extracellular medium reduces toxic metal accumulation in Pseudomonas aeruginosa and increases bacterial metal tolerance. Environ Microbiol. 2: 419 – 425

Microbial communities- Cambridge


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