Genomics. What can DNA tell you?. Tools for handling and analyzing DNA. Restriction endonucleases PCR Separation technologies Hybridization RFLP analysis Very useful for analyzing specific regions of DNA Technologies developed during 1970s and 1980s. Genomics: the bigger picture.
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What can DNA tell you?
First “minisatellite” DNA
“Father of DNA fingerprinting”
See story on p. 312 of textbook
Blood stain compared
to seven different people
Which one matches?
In situ hybridization
You can find the location
of sequences on
chromosomes this way
How can you apply genome comparison?
Organisms that look
alike may not necessarily
Epidemiology: where did a disease-causing organism come from?
Chromosomal- which band
Linkage- which genes are inherited together
Physical- where exactly are the genes located