Gene expression in early stages of micorrhizal interactions
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Gene Expression in Early Stages of Micorrhizal Interactions. By Sahel Arora. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae -- hyphae penetrates the outer cells of plant root. What is Mycorrhizae - review.

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Gene Expression in Early Stages of Micorrhizal Interactions

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Gene expression in early stages of micorrhizal interactions

Gene Expression in Early Stages of Micorrhizal Interactions

By

Sahel Arora


What is mycorrhizae review

a mutualistic symbiosis between plant and fungus

2 major types

Endomycorrhizae-- fungal component are Glomales

Endomycorrhizae --hyphae penetrates the outer cells of plant root

What is Mycorrhizae - review


Review ectomycorrhizae

Review -- Ectomycorrhizae

  • associated plant species include majority of vascular plants

  • Essential for health and survival of conifers and hardwood species

  • the function depends on the ability of the fungal symbionts to take up nutrients


Ectomycorrhizal organ

Ectomycorrhizal Organ

  • Formation has 4 stages

  • Time of the process varies

  • Laccaria bicolor – Pinus resinosa interaction takes eight weeks

  • Pisolithus tinctorius – Eucalyptus globus takes about two weeks


Fungal gene expression in early symbiotic interactions between laccaria bicolor and red pine

Fungal gene expression in early symbiotic interactions between Laccaria bicolor and red pine

  • Authors – G.K. Podila, J. Zheng, S. Balasubramaniam, S. Sundaram, S. Hiremath, J.H. Brand, and M.K. Hymes

  • Aim – to obtain a broad overview on various genes involved in the pre-infection stages of ectomycorrhizal formation, in the fungal partner

  • Uses L. bicolor for the fungal component and Pinus resinosa for the plant

  • it is known that signaling genes are involved in the early stage, the types of genes are unknown


Materials and methods

Materials and Methods

  • L. Bicolor was obtained, grown and maintained on MMN medium

  • In vitro system was used to isolate and analyze genes

  • cDNA library was constructed

  • cDNA – complimentary DNA; synthesized by using reverse transcriptase and ohage vector

  • cDNAs represent mRNAs during: a) its interaction with red pines for 6 – 72 hrs; b) free-living conditions


Materials and methods1

Materials and Methods

  • suppression subtractive hybridization was used to generate cDNAs, enriched in IS sequences

  • These sequences were used as probes to screen the actions of cDNA

  • The resulting clones were converted to plasmid clones and purified

  • The clones went through the membrane array analyses and northern analyses for validation


Results and discussion

RESULTS AND DISCUSSION

  • The analyses indicated a relative increase in the expression of each gene in the interaction over the free-living state

  • Also, showed an increase in levels of clones tested, however there was a variation

  • The data identified several regulatory genes and insights into gene activities involved


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