Gene expression in early stages of micorrhizal interactions
Sponsored Links
This presentation is the property of its rightful owner.
1 / 9

Gene Expression in Early Stages of Micorrhizal Interactions PowerPoint PPT Presentation


  • 66 Views
  • Uploaded on
  • Presentation posted in: General

Gene Expression in Early Stages of Micorrhizal Interactions. By Sahel Arora. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae -- hyphae penetrates the outer cells of plant root. What is Mycorrhizae - review.

Download Presentation

Gene Expression in Early Stages of Micorrhizal Interactions

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Gene Expression in Early Stages of Micorrhizal Interactions

By

Sahel Arora


a mutualistic symbiosis between plant and fungus

2 major types

Endomycorrhizae-- fungal component are Glomales

Endomycorrhizae --hyphae penetrates the outer cells of plant root

What is Mycorrhizae - review


Review -- Ectomycorrhizae

  • associated plant species include majority of vascular plants

  • Essential for health and survival of conifers and hardwood species

  • the function depends on the ability of the fungal symbionts to take up nutrients


Ectomycorrhizal Organ

  • Formation has 4 stages

  • Time of the process varies

  • Laccaria bicolor – Pinus resinosa interaction takes eight weeks

  • Pisolithus tinctorius – Eucalyptus globus takes about two weeks


Fungal gene expression in early symbiotic interactions between Laccaria bicolor and red pine

  • Authors – G.K. Podila, J. Zheng, S. Balasubramaniam, S. Sundaram, S. Hiremath, J.H. Brand, and M.K. Hymes

  • Aim – to obtain a broad overview on various genes involved in the pre-infection stages of ectomycorrhizal formation, in the fungal partner

  • Uses L. bicolor for the fungal component and Pinus resinosa for the plant

  • it is known that signaling genes are involved in the early stage, the types of genes are unknown


Materials and Methods

  • L. Bicolor was obtained, grown and maintained on MMN medium

  • In vitro system was used to isolate and analyze genes

  • cDNA library was constructed

  • cDNA – complimentary DNA; synthesized by using reverse transcriptase and ohage vector

  • cDNAs represent mRNAs during: a) its interaction with red pines for 6 – 72 hrs; b) free-living conditions


Materials and Methods

  • suppression subtractive hybridization was used to generate cDNAs, enriched in IS sequences

  • These sequences were used as probes to screen the actions of cDNA

  • The resulting clones were converted to plasmid clones and purified

  • The clones went through the membrane array analyses and northern analyses for validation


RESULTS AND DISCUSSION

  • The analyses indicated a relative increase in the expression of each gene in the interaction over the free-living state

  • Also, showed an increase in levels of clones tested, however there was a variation

  • The data identified several regulatory genes and insights into gene activities involved


  • Login