University of Maryland Eastern Shore

1. University of Maryland Eastern Shore Hazard Communication ProgramChemical Hygiene Laboratory SafetyCampus Emergency ProceduresCampus Regulatory Procedure

2. UMES Website

3. UMES Written Hazard Communication Program (HCP) • Fulfills the requirements set forth in the OSHA Hazard Communication Standard (H.C.S.), 29 CFR 1910.1200 will be met in this laboratory. • Right-to-Know Program • Material Safety Data Sheets • www.umes.edu/EHS

4. Purpose • Teaches the student/staff to protect themselves • Reduces the number of accidents by promoting safe laboratory practices

5. UMES Training Office • Environmental Health and Safety Office • HTTP://www.umes.edu/EHS • Located in Physical Plant • Personnel • Mr. Preston Cottman, Health and Safety Manager • Ext. 6552 Email plcottman@umes.edu • Ms. Jicola Sturgis, Assistant Health and Safety Manager • Ext. 6552 Email jrsturgis@umes.edu

6. Department of Natural Science Trainers • Ms. Amelia Potter • Laboratory Coordinator and Lecturer • Hazardous Communication Regulatory Trainer • My contact information is: • Office: CH 2105 • Email: agpotter@umes.edu • Phone: Ext. 6044

7. Hazard Communication Plan

8. Right-to-Know Video Training • Please play the Right-to-Know Video at this time.

9. Hazards • Health Hazards • Exposure • Biologicals • Blood • Other Potentially Infectious Materials (OPIM) • Chemicals • Physical Hazards • Chemicals • Reactivity, spills • Fire / Burns • Clutter • Cold Traps/Cryo • Gas/Cylinders • Chemical Storage • Sharps • Equipment

10. Hazardous Chemicals • Physical Hazards • Flammables • Reactive Chemicals • Oxidizers • Pyrophoric • Col traps cryogens • Compressed gases • Corrosives • Incompatible Chemicals • Light sensitive chemicals • Peroxide-forming • Health Hazards • Carcinogens (formaldehyde) • Toxins, Poisons • Irritants • Sensitizers • Reproductive Toxins • CNS toxins • Agents organ damage

11. MSDS • Ethanol, Anhydrous (Histological), Fisher Chemical - 681075 - Chemical Product Phy.Prop Display - Fisher Scientific

12. MSDS • Hydrochloric Acid Fisher Chemical -

13. The Material Safety Data SheetsLOCATION • Where should MSDSs be located in lab: • Hanging Folder/Binder by the laboratory door. • Copy at a predetermined location. • Where to get a MSDS to put in Binders: • Shipped with Chemicals to person who placed order. Sometimes goes to EHS • ONLINE from Chemical Company • (Note that MSDSs from different companies are different and present different information)

14. The Material Safety Data SheetsResponsibility • Teaching Laboratories: Faculty Instructor (FI) is responsible for insuring that these documents are present and are up to date. • Research Laboratories: Principle Investigator (PI) is responsible for insuring that these documents are present and are up to date. • If they are not, please contact EHS and provide the building, the room, and the PIs name.

15. Hazardous Materials Handler Training • Persons who handle hazardous chemical or biological materials and/or waste must be trained. • Chemical Hazards • Hazardous Materials Committee: • Mr. Preston Cottman - EHS • Biological Hazards • Biosafety Committee: • Dr. Victor Xsia, Pharmacy

16. Hazardous Material Storage • All Hazardous material, including waste, must be stored securely. • This includes locking the materials, including waste, such that unauthorized persons do not have access. • Do not store mixed hazards. Separate flammables, corrosives, metals and others.

17. Chemical Hazards • Hazardous chemicals are those that will cause injury to humans, animals or the environment. • These include those classified as flammable, corrosive (acids or bases), irritants, toxins, carcinogens, mutagens, teratogens, oxidizers, explosives, light sensitive, cryo, peroxide, compressed gases, etc.

18. Chemical Hazards • It also includes those that can also cause severe environmental damage, such as drugs and hormones.

19. AREA NOTIFICATION • Anyone entering as part of work or research must be trained concerning the hazards in their workplace. • All labs using hazardous chemicals should have the chemical in use posted on the door and limited access.

20. Hazardous ChemicalHandling, Storage and Waste • Handle, store and dispose of hazardous chemicals as described in the MSDS. • All chemicals, solutions and waste must be properly labeled. • Do not store mixed hazards. Separate flammables, corrosives, metals, . . . .

21. Hazardous Material Labeling Labeling of Solutions: • All of the solutions which you use should be labeled. • A proper label be legible and will state, at minimum, • the name and concentration of the chemicals in the solution, • the date, (mm/dd/yyyy) • the PIs full name • Your full name. • The generating grant no.(###-###-…) or the generating class (ENVS 101) DO NOT remove or deface label

22. Fire Protection Label • Rating • 4 most Hazardous • 0 no Hazard • Red = Flammability • Yellow = Reactivity • Blue = Health • White = Personal Protection Equipment/ Other

23. Hazardous Material Transfer Transfer of Solids: • Wear Gloves and Goggles. • Always handle bottles PALM to LABEL. • Pour an amount from the labeled dispensing stock bottle into a labeled beaker. • Then transfer from the beaker the amount needed to your receptacle. • Do not pour the unused contents of your beaker solution back in the dispensing bottle. Use a labeled recycle bottle or waste bottle.

24. Hazardous Material Transfer Transfer of Liquids and Solutions: • Wear Gloves and Goggles. • Always pour a solution PALM to LABEL. • Pour liquids/solutions from the labeled dispensing stock bottle into a labeled beaker. • Then pour from the beaker to your graduated cylinder or pipet the amount needed. • Do not pour the unused contents of your beaker solution back in the dispensing bottle. Use a labeled recycle bottle or waste bottle.

25. Handling CORROSIVES (Acids/Bases) • Concentrated solutions of acids and bases must be used in a Chemical Fume Hood. Note that these are not HEPA filtered biological hoods, used to culture cells. Never breath acid or base fumes. • Acids and Bases should be washed off immediately with water. Check for burns. • Add Acid into Water to disperse the heat when diluting. • Store in Corrosive cabinets. • Do not store with metals nor flammables.

26. Handling Flammables • Flammables should be used in a Chemical Fume Hood with non-sparking hot plates and motors. • No Smoking signs should be posted. • Note that the vapor of some organics may burn, such as ethanol. These should be extinguished with the proper foam-based fire extinguisher. Do not disperse the vapor. • Flammables must be stored in flammable cabinets, • separate from corrosives and metals.

27. Handling Explosives • Explosives should be used in explosion proof rooms and in the proper environment. • Explosives must be stored under the proper environment, in explosive retardant cabinets, in explosion proof rooms, separate from corrosives and metals.

28. Handling Irritants, Toxins, etc. • Both the vapor and the solid/liquid material of these chemicals should be avoided. • Wear gloves and goggles, with breathing apparatus when instructed in MSDS. Do not breath in nor touch skin. • Store and dispose according to MSDS.

29. Chemical Spills Chemical Spills : • In the event of a chemical spill, follow the instructions in the MSDS. • Notify the PI/Faculty of the spill. • Notify EHS of the spill.

30. Signs of Chemical Exposure • Headache • Muscle Aches • Dizzy • Burning in eyes, nose, throat • Sick • Seizure • Get to ventilated area immediately. Seek medical attention.

31. HAZARDOUS CHEMICAL WASTE DISPOSAL • Disposal procedure and the label must follow the instructions provided for Hazardous Chemical Waste Disposal located on the EHS website: • www.umes.edu/EHS • Large Quantity Generator Rules apply. • Label all hazardous chemical waste bottles, with the appropriate EHS label prior to conducting the experiment.

32. REMINDERS for WASTE • Store securely. Store in a locked area. Fume hoods are NOT storage areas. • Dispose of Solids and Liquids Separately • Containers should be compatible for waste material. • Bottles should be sealed. DO NOT use aluminum foil, parafilm nor corks to seal bottles or containers.

33. HAZARDOUS CHEMICAL WASTE DISPOSAL Procedure Summary • Each bottle should be labeled with a yellow 4in X 4in label printed with the EHS logo. Template to use Avery label (use two 2” X 4”) is available. • Completed Waste Disposal Form should be completed • MSDS should be provided for each chemical. • (see EHS website).

34. HAZARDOUS CHEMICAL WASTE DISPOSAL Procedure Summary 2 • PI or FI are the generators and should submit waste to EHS for pick up Quarterly or when storage area is full.

35. EHS Website

36. Waste Disposal Procedure Webpage

37. Chemical Waste Disposal

39. Biosafety Program Website

40. BIOLOGICAL HAZARDSDefinitions • Hazardous biologics are those that will cause injury to humans, animals or the environment. • These are classified by Biosafety Level (BSL), which refers to the mode of transport and the level of infection. • BSL 1 is a normally occurring biological at environmental levels which may cause limited pathogenesis in humans, animals or plants. BSL 1 are allowable on campus.

41. BSL 1 Definition

42. BIOLOGICAL HAZARDS • BSL 2 are classified as having potential for pathogenesis but not airborne transport. Low potential for vector infection or death. UMES is working toward a BSL 2 lab. We cannot order BSL 2 biologics to date? • BSL 3 are classified as being pathogenic and airborne transport. High potential for vector infection. These biologics usually result in death. We cannot order BSL 3 or higher biologics to date.

44. AREA BSL NOTIFICATION • Anyone entering as part of work or research must be trained concerning the hazards in their workplace. • All labs using biologics should have the level of the biologic posted on the door and limited access. • Yellow BIOSAFETY warning notices, for research with BSL 2 or higher, must be posted on the exterior door.

45. HANDLING HAZARDOUS BIOLOGICS • Ordering of BSL materials must be approved by the EHS and the BSC. • BSL 1 require minimum protection of gloves and goggles. • BSL 2 require limited access and exposure and should not be used without prior instruction from EHS and the UMES Biosafety committee. • BSL 3 and higher are not allowed on campus at this time.

46. BIOSAFETY DISINFECTION • Surfaces used with biologics must be disinfected by the method described in the MSDS. • At a minimum, this includes the use of a disinfecting compound or device, such as UV light. • Gloves, even unused, may not be worn outside the laboratory.

47. HAZARDOUS BIOLOGICAL WASTE DISPOSAL • Biologics, including media, and cells, should be autoclaved before disposal. • Use a regular PE bag and autoclave tape to autoclave BSL 1 level biologics and trash. • Use a sealed box inside a regular PE bag and autoclave tape to autoclave BSL 1 spent needles and sharps. • The BIOSAFETY orange autoclave bags and/or sharps disposal boxes are reserved for use with BSL2 and higher.

48. HAZARDOUS BIOLOGICAL WASTE DISPOSAL • Disposal procedure and the label must follow the instructions provided for Hazardous Biological Waste Disposal located on the EHS website: • www.umes.edu/EHS • Label all hazardous chemical waste bottles, with the appropriate EHS label prior to conducting the experiment.

49. Biological Waste Disposal Procedure

50. Charge for Waste Disposal • Your grant/department will be charged for all hazardous waste disposal. This may be included in the overhead of grants. • Unknown waste costs $4000/bottle. Please label materials and waste. • It is a violation to “dump” your waste in another area. It must disposed from the area from which it was generated.