1 / 24

R.I.P. Steve Jobs

R.I.P. Steve Jobs. Hao Li Oct.7 th 2011. Social Defeat Paradigm. Long–Evans rats (325–350 g). Sprague–Dawley rats(250–275 g). V.S. Social Defeat Paradigm. Social Defeat Paradigm. 5 min fighting or until the submission Separated with wire mesh cage for 15 min 4 consecutive encounters

cate
Download Presentation

R.I.P. Steve Jobs

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. R.I.P. Steve Jobs

  2. Hao Li Oct.7th 2011

  3. Social Defeat Paradigm Long–Evans rats (325–350 g) Sprague–Dawley rats(250–275 g) V.S. Social Defeat Paradigm

  4. Social Defeat Paradigm • 5 min fighting or until the submission • Separated with wire mesh cage for 15 min • 4 consecutive encounters • 15 min between each encounters

  5. Depressive-like Behaviors • Sucrose preference test----Anhedonia • Forced swim test----Behavioral Despair • Contextual fear test----Memory • Social approach and avoidance test

  6. Sucrose preference test 2 bottles water for 5 days Increasing concentrations of sucrose (0.25–1%) for 2 days per concentration

  7. Contextual fear test • For the memory of traumatic event • Putting the rats and an aggressor in the same cage for 5 min • Measuring the freezing time

  8. Social approach and avoidance • 3 sections each for 5 min • Section 1- free walks in the open field • Section 2- “no target” • Section 3- “target” • Measuring the locomotion and investigating time

  9. Histone Modification • Histone acetylation makes RNA polymerase and transcription factors easier to access the promoter region.

  10. Histone Modification • Histone H3 • Histone H4

  11. Histone Modification Upregulation of BDNF Transcription Downregulation of c-fos Transcription (Tsankova 2004) • H3 Acetylation Electroconvulsive Seizures • H4 Acetylation

  12. Behavioral Tests Sucrose Preference Test: Both defeated and control rats similarly preferred increasing sucrose concentrations over water.

  13. Behavioral Tests Forced Swim Test:

  14. Behavioral Tests Social approach and avoidance test Section 2 Section 1

  15. Behavioral Tests Section 3

  16. Behavioral Tests Contextual fear test: 4 weeks 6 weeks

  17. Sucrose preference test Forced swim test Contextual fear test Social approach and avoidance test Depressive-like Behaviors

  18. Histone Modification • No significant changes in H3 acetylation in amygdala and dorsal profrontal cortex amygdala dorsal prefrontal cortex the ratio of histone H3 acetylation (H3hyp) to total histone H3 (TH3)

  19. Histone Modification hippocampus

  20. No changes in H4 acetylation and H3 acetylation in the amygdala or dorsal prefrontal cortex ----Highly heterogeneous region ----Lack of antibody specificity

  21. Histone Modification • Two potential pathways in histone acetylation: N-methyl-D-aspartic acid receptor (NMDA-R) glucocorticoid receptor (GR)

  22. MK-801----increasing acetylation • Mifepristone----no significant changes Former study showed both of them blocked the acetylation (Chandramohan Y 2007) Different changes to different stressors

  23. Thanks…

More Related