Ivermectin Effects on Caenorhabditis elegans C.elegans

Ivermectin Effects on Caenorhabditis elegans C.elegans PowerPoint PPT Presentation


  • 288 Views
  • Uploaded on
  • Presentation posted in: General

Background Information. . 1.Anti-parasitic MedicationStrongyloides stercoralis Onchocerciasis volvulus Pubic lice infestation Scabies Wuchereria bancrofti . 2 Mechanism of action Bind to GABA Channel Hyperpolarization by ?Cl Permeability. http://www.pnas.org/cgi/content/full/97/6/2674/F2.

Download Presentation

Ivermectin Effects on Caenorhabditis elegans C.elegans

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


1. Ivermectin Effects on Caenorhabditis elegans (C.elegans)

2. Background Information

3. Background Information Phylum Nematoda Feed on OP50 bacteria Pseudocoelomates Thrashing motion (contraction) Female deposit 100,000 or more egg/day Most individuals are Hermaphrodites Life Cycle : >12hours

4. Research Purpose :To determine the effect of Ivermectin on wild type (C.elegans) Plan to extend strain Complications Method of introduction Drug Test Resistance strain

5. Ivermectin response to OP50 bacteria Ivermectin has no effect on OP-50 bacteria

6. Drug Introduction

7. Wild type C.elegans

8. Ivermectin: Wildtype worm

9. Comparison

10. Touch insensitive=Tapping

11. Future Analysis Growth Assay: Count the number of gravid adult Reflects Pharyngeal function Mechanosensation: Touch sensitivity Neurological Response Motility assay: Number of worm still thrashing Locomotion

12. References American Society of, Health-System Pharmacists Inc. Patient Drug Information From MedMaster. Maryland: 2007. Jamison, Dean T., and Joel G. Breman. Disease Control Priorities in Developing Countries. 2nd. New York: Oxford University Press, 1993. John Keane and Leon Avery Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genes. Genetics. 2003 May; 164(1): 153–162. PMCID: PMC1462566 Griffiths, Anthony J.F., and Jeffery H. Miller. HumanGenetics. 7th ed. New York: W.H. Freeman,1996. Hart, Anne C. "Behavior." Worm Book July 3 2006. 04 April 2008 <http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=wormbook. capter.7133>.

  • Login