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Guidance of neuronal growth with DEP

Guidance of neuronal growth with DEP. J. James Jun Presenter University of Calgary Dr. Rudolf Potucek Supervisor University of Calgary January 25, 2007. Application of dielectrophoresis(DEP) for directing neuronal growth. Neuronal Guidance. Theory of DEP. Modeling & Experiment.

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Guidance of neuronal growth with DEP

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  1. Guidance of neuronal growth with DEP J. James Jun Presenter University of Calgary Dr. Rudolf Potucek Supervisor University of Calgary January 25, 2007

  2. Application of dielectrophoresis(DEP) for directing neuronal growth Neuronal Guidance Theory of DEP Modeling & Experiment

  3. Neuronal growth: random vs. directed Directed Random

  4. Electric forces: Electrophoresis vs Dielectrophoresis Electrophoresis Any electric fields Charged object DC Dielectrophoresis Spatially varying field Polarizable object DC or AC

  5. Modeling and Experiment Neuron Pond snail Brain Microsphere Field Modeling Vision Tracking

  6. Experiment with polymer bead

  7. Electric field modelingQuadruple electrodes configuration top side

  8. Computer vision tracking

  9. Experiment with a live snail neuronChallenges More complicated than the experiment with beads Long term experiment (3 days) Need an evaporation control with perfusion system Need a neurotrophic factor enriched medium

  10. ConclusionsDEP can be used to direct a neuronal growth with electric field modeling and computer vision tracking DEP is a non-contact force and bio-chemically safe Electric field modeling to optimizes DEP parameters Computer vision tracking to track a growthcone Experiments planned with beads then snail neurons Questions?

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