Genetical features of chromosomal form c of microtus maximowiczii from the russian far east
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GENETICAL FEATURES OF CHROMOSOMAL FORM « C » OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST. Irina V. Kartavtseva, Irina N. Sheremetyeva Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia) Anastasia B. Ryabkova

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Genetical features of chromosomal form c of microtus maximowiczii from the russian far east

GENETICAL FEATURES OF CHROMOSOMAL FORM«C» OF MICROTUS MAXIMOWICZII FROM THE RUSSIAN FAR EAST

Irina V. Kartavtseva, Irina N. Sheremetyeva

Institute of Biology & Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok (Russia)

Anastasia B. Ryabkova

Khabarovsk Antiplague Station, (Russia)

Svetlana A. Romanenko, Olga L. Gladkikh

Institute of Molecular and Cellular Biology SB RAS (Russia)

Lubov V. Frisman

Institute of Complex Analysis of Regional Problems Far Eastern Branch of Russian Academy of Sciences, Birobidzhan (Russia)


The role of chromosomal rearrangements in speciation still unclearly

The role of chromosomal rearrangements in speciation still unclearly.

Introduction

The maximowicz's vole Microtus maximowiczii (Rodentia) is convenient species for investigating this problem.


Irina v kartavtseva irina n sheremetyeva

All rearrangements for this species can be divided into three types:

  • Rearrangements by Mejer et al., 1996

1 - is fusion of two middle sized pairs of metacentrics to formating of large metacentric.

2 - is Robertsonian rearrangements.

3 - is pericentric inversions of 4 pairs.


Areal and terra typica points of m maximowiczii

Areal and terra typica points of M. maximowiczii

Russia

M. m. maximowicziiSchrenck 1858,

Amurskii and Khabarovskii regions in Russia, and in northeastern China

M. m. ungurensisKastchenko 1913,

Buryatia and Chitinskii regions in Russia and North-Eastern Mongolia

Mongolia

China

Korea


For this species was described five chromosomal forms kartavtseva et al 2008

For this species was described five chromosomal forms (Kartavtseva et al., 2008):

  • A : 2n = 39 - 44, NF = 53 - 60 (Buryatiya),

  • B: 2n = 38 - 42, NF = 54 - 57 (Buryatiya),

  • C: 2n = 39 - 41, NF = 55 - 60 (Far East)

  • D: 2n= 40=43, NF= 54-58 (Zabaikalskii kray and E. Mongolia)

  • V: 2n = 36 - 40, NF = 52 - 56 (Transbaikalia and W.Mongolia).


Irina v kartavtseva irina n sheremetyeva

5 chromosomal forms of M. maximowiczii (Kartavtseva et al., 2008)

«B»

2n=38-42

«A»

2n=39-44

Russia

M. m. ungurensis

M. m. maximowiczii

Mongolia

«D»

2n=40-43

«C»

2n=39-41

«V»

2n=40-43

China


Irina v kartavtseva irina n sheremetyeva

"A“

2n=39-44

M. m. ungurensis

M. m. maximowiczii

"C“

2n=39-41


Irina v kartavtseva irina n sheremetyeva

control region mtDNA

Mmax45

83

Mmax41

78

Mmax38

45

Mmax39

98

42

Mmax42

68

Mmax40

Mmax37

55

Mmax43

Mmax44

65

25

Mmax47

45

Mmax33

79

Mmax48

Mmax51

78

Mmax52

74

Mmax49

59

52

Mmax50

34

Mmax33

93

65

Mmax34

62

Mmax36

Mmax35

Mmax8

80

96

29

Mmax11

Mmax7

50

Mmax9

50

Mmax10

92

Mmax30

Mmax29

74

95

Mmax25

Mmax26

73

Mmax27

69

Mmax31

50

Mmax28

47

Mmax32

31

Mmax1

68

Mmax2

51

Mmax4

Mmax5

87

Mmax3

83

Mmax6

Mmax16

98

Mmax17

76

76

Mmax18

Mmax24

Mmax14

Mmax22

55

Mmax13

Mmax12

Mmax15

98

Mmax23

Mmax21

Mmax19

13

Mmax20

Microtus oeconomus

0.01

M. m. maximowiczii

M. m. ungurensis

С

“Восточная”

А

D

“Южная”

B

“Западная”

V


Material map of chromosomal investigation

MaterialMap of chromosomal investigation

We investigated karyotype of 36 individuals of Maximowicz's vole from six earlier not investigated populations of the territory Middle Amur river (chromosomal form C).

Literature data:

  • 2n=42

  • 2n=39

    Our data:

  • (2n=40-41)

  • New data


2n 40 41

2n=40-41

2n=41

2n=40


Chromosomal forms d and c

Chromosomal forms “D” and “C”

2n=42

2n=40


New chromosomal nomenclature of m maximowiczii by fluorescent in situ hybridization fish

New chromosomal nomenclature of M. maximowiczii by fluorescent in situ hybridization (FISH)

20

M. maximowiczii (#2350)

from Romanovka

(chromosomal form “A”, our data)

11,20

11

2n=41

M. maximowiczii FISH by Lemskaja et al., 2010

from chromosomal form “A”


M maximowiczii 2060 from norskii reservation our data

M. Maximowiczii (#2060)from Norskii reservation (our data)

11

20

20

11


Irina v kartavtseva irina n sheremetyeva

Map of investigation of control region A Bmt DNA 940 np

  • A

A

B


Conclusion

Conclusion

In the territory Middle Amur river (chromosomal form C):

  • Stabilization of karyotypewith Robertsonian rearrangement chromosomal pairs 11 and 20 and inversion of pair 14

  • Two clasters of control region of mt DNA can be results of isolations of two animals groups.


Thanks for attention

Thanks for attention


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