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Sulfate-reducing bacteria. Derya Ozuolmez HMC 2012. Sulfate reducers,. Strict anaerobes , Belong to 4 phylogenetic lineages; Delta- proteobacteria , thermophilic Gram (-) bacteria , Gram (+) bacteria, Euryarchaeota Reduce sulfate to sulfide ,

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Sulfate reducing bacteria

Sulfate-reducing bacteria

DeryaOzuolmez

HMC 2012


Sulfate reducers
Sulfate reducers,

  • Strict anaerobes,

  • Belong to 4 phylogenetic lineages;

    • Delta-proteobacteria, thermophilicGram (-) bacteria, Gram (+) bacteria, Euryarchaeota

  • Reduce sulfate to sulfide,

  • Electron donors (H2, HCOO-, acetate, butyrate, propionate, ethanol)

  • Two physiological groups of SRB;

    • Incomplete oxidizers: organic compounds to CO2 and acetate

    • Complete oxidizers: organic compounds to CO2


Starting enrichments
Starting enrichments


Enrichments
Enrichments

  • Sediment sample in 50 ml Pfennig bottle

  • Incubated @ 30oC

  • Checked regularly for turbidity, blackening, H2S smell…

  • Observed microscopically

  • 3 ml culture was transferred to a new bottle for further enrichment.



Isolation step
Isolation step…

1 ml

culture

3ml 3% agar + 6 ml medium

SRB enrichment

culture

The headspace gas was exchanged by

purging with N2 during 1 minute.


Some nice agar shakes
Some nice agar shakes…

By courtesy of Katrin & Jose!!!)


Thank you!

Questions?


Activation of sulfate
Activation of sulfate

2[H]

6[H]

ATP

SO42-

APS

HSO3-

HS-

ATP sulfurylase

APS reductase

Bisulfite reductase (dsr)

PPi

Pyrophosphate


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