1 / 15

Frank Greenway, M.D. Pennington Center March 3, 2009

Frank Greenway, M.D. Pennington Center March 3, 2009. Angiogenesis. Angiogenesis, growing new blood vessels, is a basic biological process Angiogenesis in adults is used for menses, fetal development and healing wounds.

cammiec
Download Presentation

Frank Greenway, M.D. Pennington Center March 3, 2009

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Frank Greenway, M.D. Pennington Center March 3, 2009

  2. Angiogenesis • Angiogenesis, growing new blood vessels, is a basic biological process • Angiogenesis in adults is used for menses, fetal development and healing wounds. • Inhibition of angiogenesis in adults should have minimal side effects or toxicity. • Cancer cannot grow past 2mm without angiogenesis and fat needs angiogenesis too.

  3. Human Angiogenesis Assay • Discs are cut from the veins of placentas (after births). These placentas are left over after the delivery of a baby and would be discarded. The discs are put in a clot-like gel. • The discs sprout new blood vessels (initiation) and the new vessels continue to gro over a 2-week period (angiogenic index from 1-16)

  4. Human Tissue Predicts Activity Jung SP et al. Angiogenesis. 4(3):175-86, 2001. Hornick CA et al. Angiogenesis. 6(2):143-9, 2003.

  5. Sweet Leaf Tea • This is a different tea than tea from the plant Camilla, the tea consumed in Britain, the US (black) or in Chinese restaurants (green) • Sweet leaf tea is sweet due to a chemical made by the plant called Ruboside that is a non-caloric sweetener. • Sweet leaf tea is consumed as part of the diet in an area in China and is related to blackberry, a plant called Rubus.

  6. Black Raspberry

  7. Inhibition of Angiogenesis by Sweet Leaf Tea in placental vein assay

  8. Fat is an Angiogenic Tissue • Fat grows and recedes throughout life and is composed basically of fat and vessels. • TNP-470 is a fungus-derived angiogenic inhibitor that makes people very sick. • TNP-470 and other angiogenic inhibitors have been shown to reverse obesity in rodent models of obesity and it does not make the rodents sick.

  9. Intermittent Treatment with TNP-470, 10 mg/kg/d

  10. Human Angiogenesis Assay in Fat Tissue

  11. Active Ingredient .001 M 0.0001 M Control

  12. Sweet Leaf Tea Inhibits Angiogenesis in Human Fat Tissue

  13. p 0.40 RUO-C x 0.35 0.30 q dd v w 0.25 u AU r 0.20 t o s 0.15 n h cc gg y ee ff 0.10 a d z bb c j m hh kk f aa ii i k l jj b e g 0.05 0.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 Minutes Crude Extract with 26 Chemicals

  14. Partially Purified Extract with 13 Chemicals

  15. Purification and Standardization • Purification gets rid of the plant chemicals and other plant components that do not inhibit angiogenesis. Purification resulted in 5 chemicals important for effectiveness • Standardization is the identification of the important chemicals and being sure that every batch of the purified plant has the full amount of the effective chemicals. Standardization is necessary for a consistent product.

More Related