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Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence

“ FIRST ” generation sequencing. Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence. carry out dideoxy sequencing. connect seqs. to make whole chromosomes. find the genes!.

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Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence

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  1. “FIRST” generation sequencing Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes find the genes!

  2. M.L. Metzker 2010. Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs. Ion torrent Nanopore

  3. 5 Selected Technologies. 2nd, 3rd, and 4th generation sequencing.Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, 4327. • Solexa - - ->Illumina

  4. Allows both directions: “pair-end”

  5. Life: Roche/454; Polonator • Soon to be bought by Thermo Fischer

  6. NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

  7. Ion Torrent (BIU) • http://www.iontorrent.com/1-trillion-front-end/

  8. Pacific Biosciences

  9. New Generation: Template With hairpins On each end

  10. Nanopore DNA sequencing

  11. http://lifetech-it.hosted.jivesoftware.com/videos/1016 • ??? work

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