M wt d thya
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A. B. M wt D asd. M wt D thyA. 1564. 1829. 701. 395. 322. Supplementary Fig. 1. Molecular characterization of S. typhimurium strains by colony PCR. a) An asd primer set was used

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M wt D thyA

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M wt d thya

A

B

M wt Dasd

M wt DthyA

1564

1829

701

395

322

Supplementary Fig. 1.

Molecular characterization of S. typhimurium strains by colony PCR. a) An asd primer set was used

for PCR with strain SL7207 (wt) and strain c8799 (Dasd) that encompasses the deletion. b) A thyA

primer set was used for PCR with strain SL7207 (wt) and strain SL7207DthyA that encompasses the

disruption. Due to the strategy of disruption the two amplification products observed are expected.

Numbers indicate sizes of PCR products in bp. M indicates size marker: FX174 HaeIII.


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