Lab 2 : Food Sampling & Preparation of Sample Homogenate. Food Sampling & Preparation of Sample Homogenate. The adequacy and condition of the sample or specimen received for examination are of primary importance.
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Lab 2:Food Sampling & Preparation of Sample Homogenate
plastic bottles are useful containers for line samples.
marked strip of masking tape.
until arrival at the laboratory.
analyzed more than 36 h after collection.
1. A sample, consisting of a specified number of sample units (usually five) drawn at random from each lot, shall be taken.
2. Each sample unit shall consist of at least 100 ml or g, unless stipulated in the method.
3. Collect original unopened container wherever possible.
4- If the product is in bulk, several sample units can be collected from one container, while ensuring that the total number of sample units are not collected from one container.
from large institutional or bulk containers when
the total number of sample units required exceeds
the number of container in the lot.
to thaw during shipment.
(a) that quantity of product produced under essentially the same conditions, at the same establishment and representing no more than one day's production; or,
(b) the quantity of the same kind of product from one and the same manufacturer available for sampling at a fixed location.
the product, and should consist of a minimum of 100 g (ml),
unless stipulated in the method.
On HGMF the Growth Unit (GU) is equivalent to the more
familiar Colony Forming Unit (CFU).
of above defects would invalidate the sample, and
the collecting district should be notified.
Collection Report and officially sealed with tape
bearing the sample number, collecting official's
name, and date.
than 45°C for not more than 15 min.
sample continuously in thermostatically controlled
dried samples with sterile spoons or other utensils before withdrawing the analytical unit from a sample of 100 g or greater.
appropriate Bacteriological Analytical Manual method being used.
(e.g., a frozen dinner), macerate entire contents of package and
withdraw the analytical unit, or, preferably, analyze each different
food portion separately, depending on purpose of test.
to deliver 1 ml volumes; for delivering 0.1 ml volumes,
do not use pipet with capacity greater than 1.0 ml.
END OF LECTURE