MICR 304 Immunology & Serology. Lecture 8A Antibodies Part II Chapter 4.2- 4.5, 4.12 – 4.20, 9.14 – 9.20. Overview of Today’s Lecture. Generation of antibodies Secondary antibody modifications Effector functions of antibodies. Key Players in Immunology.
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Antibodies Part II
Chapter 4.2- 4.5, 4.12 – 4.20, 9.14 – 9.20
L: Leader sequence; directs protein to cell’s secretory pathway
: Hinge region
on chromosome 22
on chromosome 2
on chromosome 14
Includes a significant number of non-functional segments (pseudogenes)
In heavy chain, V cannot be directly joined with J.
Involves heavy chain only
Involves variable region on heavy and light chain
Mutations in the framework tend to disrupt the antibody structure and are selected against.
IgM Pentamer (5mer)
Monomers are cross-linked by disulfide bridges that connect each other and J-chain
IgA Dimer (2mer)
IL4 induces IgE Regions
IL5 augments IgA
IFNg induces IgG
TNFb induces IgG and IgACytokine-Induced Switching of Isotypes
In conjunction with complement
Antigen:Antibody complexes Regions
Antibody conformation change
Activate classical pathway of complement
Binding of C1q to the Fc piece
Pentameric IgM is more efficientComplement Activation
Antibody Dependent Cell Mediated Cytotoxicity