Bacteria stress response to high pressure processing
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Bacteria stress response to high pressure processing. Tine Bergitte Heggernes Supervisor: Nadav Skjøndal -Bar Project with Nofima – Måltidets Hus, Stavanger. Motivation. Listeria monocytogenes. Objective and methodology. Objective

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Bacteria stress response to high pressure processing

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Bacteria stress response to high pressure processing

Bacteria stress responseto high pressure processing

Tine Bergitte Heggernes

Supervisor: NadavSkjøndal-Bar

Project withNofima – Måltidets Hus, Stavanger

Project Autumn 2013, Tine Bergitte Heggernes


Bacteria stress response to high pressure processing

Motivation

Listeria monocytogenes

Tine Bergitte Heggernes


Objective and methodology

Objective and methodology

Objective

  • Doing a literature study on bacteria stress response to high pressure processing (HPP).

  • The bacteria response - change of gene expressions and mechanisms in the cell when stressed.

    Methodology

  • Using experimental data done with HPP at 400 Mpa and 600 Mpa with Listeria contaminated food

  • Find vital HPP affected mechanisms  Genes activating these mechanisms  Determination of most significant genes  Comparing genes with regulation from other environmental stresses  Proposing the most pressure specific genes

Bacteria stress responce to high pressure processing


Results and conclusion

Results and conclusion

HPP induced changes

Many genes expressed as a response to HPP

Expression change higher at 400MPa than 600 Mpa less developed response systems for higher pressures.

Tine Bergitte Heggernes


The 7 most pressure specific genes

The 7 most pressurespecific genes

Tine Bergitte Heggernes


Futute work master thesis

Fututework – Master thesis

  • Conduct HPP experiments

  • Develop models which predicts bacteria inhibition from high pressure processing

Tine Bergitte Heggernes


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