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polimerazuli jaWvuri reaqcia realur droSi ( Real-Time PCR )

polimerazuli jaWvuri reaqcia realur droSi ( Real-Time PCR ). m. axalaia tuberkulozisa da filtvis daavadebaTa erovnuli centri, erovnuli centraluri mikobaqteriologiuri laboratoria.

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polimerazuli jaWvuri reaqcia realur droSi ( Real-Time PCR )

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  1. polimerazuli jaWvuri reaqcia realur droSi(Real-Time PCR) m. axalaiatuberkulozisa da filtvis daavadebaTa erovnuli centri, erovnuli centraluri mikobaqteriologiuri laboratoria

  2. Georgia - Vanderbilt University, Nashville, Tennessee, USA, 2006-2008 CRDF project:"Monitoring Chemotherapy Regimen Efficacy by Mycobacterium Tuberculosis mRNA Detection in Sputum Specimens” “mkurnalobis monitoringis efqturobis Sefaseba M. Tuberculosis rnm-is gamovleniT naxvelSi” saqarTvelo-vanderbildis universiteti, neSvili, tenisi, aSS, 2006- 2008, CRDF

  3. polimerazuli jaWvuri reaqciis komponentebi dnm matrica ori praimeri Termostabiluri dnm-polimeraza dezoqsinukleozidtrifosfatebi Mg 2+ ionebi buferis xsnari

  4. polimerazuli jaWvuri reaqcia denaturacia – dnm-s orjaWviani molekulis gaxleCva anilizacia – temperaturis swrafi dawevis fonze praimerebis dakavSireba erTjaWvian matricasTan elongacia – dnm-polimeraza axdens matriculi jaWvis replikacias

  5. polimerazuli jaWvuri reaqciis saxeobebi ukutranskripciuli pjr asimetriuli pjr pjr dnm-s Tavisufali boloebis swrafi amplifikaciiT raodenobrivi pjr raodenobrivi pjr realur droSi

  6. raodenobrivi pjr realur droSi principi: Cveulebrivi pjr magram: dnm/rnm molekulebis amplifikaciasTan erTad xdeba maTi raodenobis gansazRvra realur droSi TiToeuli amplifikaciis (ciklis) Semdeg

  7. amplificirebuli dnm/rnm molekulebis raodenobis gansazRvrisaTvis gamoiyeneba: fluoresenciuri saRebavebi interkalirirebuli (ukuCarTviTi) orjaWviani dnm modificirebuli dezoqsinukleotidebi, romlebic fluoresencireben dnm komplementarul fragmentebTan hibridizaciis Semdeg

  8. pjr realur droSi multirezistentuli tuberkulozis swrafi diagnostikisaTvis myar niadagze miRebuli M.Tuberculosis kulturidan; tuberkulozisswrafi diagnostikisaTvis uSualod masalidan (naxveli); tuberkulozis mkurnalobis monitoringis efeqturobis Sesafaseblad.

  9. pjr realurdroSimultirezistentuli tuberkulozis swrafi diagnostika samizne jgufi: myar niadagze (LJ) miRebuli 196 M.Tuberculosis kultura erTi testiT gamovlinda M.Tuberculosis 25 genuri mutacia, romelbic pasuxismgebelni arian INH, RIF, STM, ETM rezistentobaze

  10. multirezistentuli tuberkulozis swrafi diagnostika

  11. M.Tuberculosis swrafi diagnostika uSualod masalidan • 100 axali sadiagnozo SemTxveva • kulturalurad dadebiTi 59 SemTxveva

  12. M.Tuberculosis diagnostikismeTodebis Sedareba

  13. tuberkulozis mkurnalobis monitoringis efeqturobis Sefaseba • samizne jgufi: 65 axali sadiagnozo SemTxveva • masala Segrovda mkurnalobis dawyebidan 2 kviris, 1, 2 da 4 Tvis Semdeg • mgb baqterioskopuli gamokvleva (cil-nilseni), daTesva myar niadagze (LJ), pjr realur droSi

  14. M.Tuberculosis diagnostikismeTodebis Sedareba

  15. pjr realur droSi da daTesva myar niadagze (LJ) (meTodebisTanxmoba)

  16. dadebiTobis maCveneblis Semcireba mkurnalobis monitoringis dros

  17. uaryofiTobis maCveneblis zrda MDR da Non MDR SemTxvevebSi mkurnalobis monitoringis dros (pjr da daTesva LJ)

  18. daskvna • tuberkulozis swrafi diagnostika uSualod masalidan • multirezistentuli tuberkulozis swrafi diagnostika • maRali specifiuroba da sensitiuroba RIF da INH rezistentobis ganmsazRvreli genis mimarT • analizis Sedegs sWirdeba mxolod 2-4 saaTi rogorc gamoyofili kulturidan aseve uSualod naxvelidan

  19. Georgia - Vanderbilt University, (Nashville, Tennessee, USA), 2006-2008 CRDF project:"Monitoring Chemotherapy Regimen Efficacy by Mycobacterium Tuberculosis mRNA Detection in Sputum Specimens”. • "Simultaneous Detection of Tuberculosis Drug Resistance Mutations by a Target-Enriched Multiplex PCR" - Abstract for the 107th General Meeting of the American Society for microbiology, May 21-25, 2007, Toronto, Canada. • “Prevalence of and Molecular Basis for Tuberculosis Drug Resistance in the Republic of Georgia: Validation of a Templex System for the Detection of Drug Resistance-Related Mutations" - Antimicrobial Agents and Chemotherapy. 52:725-9.USA. • “Monitoring Therapy Efficacy by Real-Time Mycobacterium tuberculosis mRNA Detection in Sputum Specimens” - Abstract for the 108th General Meeting of the American Society for Microbiology (June 1-5, 2008, Boston, MA), American Association for Clinical Chemistry (January 23, 2009, Washington, DC). • ”DNA and mRNA-Targeted Real-Time TaqMan PCR Assays for Rapid Detection of Mycobacterium tuberculosis from Sputum Specimens” - Abstract for 39th Union World Conference on Lung Health (16-20 October 2008, Paris, France).

  20. gmadlobT yuradRebisTvis

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