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Aptamer-Development-PLATFORM

With years of ample experiences in antibody drug development, our scientists are confident in providing a full range of aptamer development services to promote the development of complement therapeutics. Creative Biolabs is proud to offers aptamer development services in a timely and cost-effective manner. We offer turn-key or ala carte services customized to our client's needs. For more, https://www.creative-biolabs.com/complement-therapeutics/aptamer.htm.

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Aptamer-Development-PLATFORM

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  1. COMPLEMENTTHERAPEUTICS Aptamer Development PLATFORM CreativeBiolabsofferscomprehensive one-stopDNA orRNAaptamer developmentservices againstdifferent targets. https://www.creative-biolabs.com/complement-therapeutics/

  2. Creative Biolabs is a leading custom service provider with extensive experience inantibodyproductionand engineering. Wehave recently builtaone-stopaptamerdevelopment platform.Weare confidentin screening aptamers withhigh affinityagainstdifferent formsoftargets,suchas smallorganic,peptide,protein,cell,and bacteria. AboutUs For more than 10 years, we have been bringing values to our valuable customersby providing high-qualityproductsand services quickly and at competitiveprices. Weprovideabroadportfoliooflaboratoryservicesforaptamer development: • One-stopAptamerInVitroSelectionService • Aptamer-basedConjugationServices • AptamerModification Services • AptamerOptimizationServices • AptamerCharacterization Services

  3. Outline 01 One-stop AptamerInVitro SelectionService 02 Aptamer-based ConjugationServices 03 AptamerModification Services 04 Aptamer Optimization Services 05 AptamerCharacterization Services 06 OurAdvantages

  4. CREATIVEBIOLABSINC. APTAMERINVITROSELECTION 1One-stopAptamerInVitroSelectionService STEP 03 STEP 02 Identification and Characterization of MonoclonalAptamer STEP 04 STEP 01 STEP 05 SelectionofPolyclonal Aptamers Produce 5 pools from phases 1 with indexed primers for NGS sequencing NGSsequencing(105to106reads per round) and identifying up to 20 sequences. Optimizationofthe BestAptamer Truncatethebestaptamer(1 aptamer) and test the binding propertiesofthevariants. Providesuggestionsfor chemical modification(s) of the best variant, suchasmodificationofthe backbone to improve the nuclease resistance; conjugationtoa fluorophore… Synthesize and purify the top 5 monoclonalaptameroligocandidate(s) Quantitativelytesteachcandidate for Kd affinities by SPR, BLI, ELISA, Microscopy, FlowCytometry,etc. LibraryEnrichment (SELEX) Chemically synthesize and modifysingle-strandedDNAlibrary or RNAlibrary Iterative roundsoftarget screening(positiveandnegative), includingsmallorganic,peptide, protein,cell,andbacteria. Deliveryand Report Supply5nanomolesofthe best monoclonal aptamer or modifiedvariant. Deliverprojectreport containing all sequences from NGSsequencing.

  5. CREATIVEBIOLABSINC. 2 Aptamer-based ConjugationServices • Becauseofthe hightargetingspecificity, aptamer-basedconjugatescan improvetheefficacyandbiologicaldistributionofmolecules, reducing the accumulationinnon-targettissuesthatcouldresultinoff-targeteffects.At CreativeBiolabs,we canconjugatevariousinteresting drugs, antibodies, nanomaterialswithaptamersand study themtoprovide effectiveand safe aptamer-basedconjugatesforresearchersworldwide. • OURAPTAMER-BASED CONJUGATEDEVELOPMENTSERVICESINCLUDE: • Aptamer-therapeuticOligonucleotide ConjugatesDevelopmentService • Aptamer-antibody ConjugatesDevelopment Service • Aptamer-drug Conjugates DevelopmentService • Aptamer-cholesterolConjugatesDevelopment Service • Aptamer-nanoparticles ConjugatesDevelopment Service • Bivalent Aptamer Development Service • Aptamer-basedBiosensor DevelopmentServices

  6. CREATIVEBIOLABSINC. • Equipped withstate-of-the-arttechnologies and richexpertisein aptamer development,we providea fullrange ofaptamer modificationservicesfor global clients.Wehavecompletedseveralchallengingaptamermodificationprojects and accumulatedextensive experience. Accordingtoclients’specificrequirements, we canoptimizeaptamermodificationsolutionstoimprovetheirperformance, such as stability or specificity. • OURAPTAMER-BASED MODIFICATIONSERVICESINCLUDE: • 2'FluoroModification • 2'-O-methylmodification • 3'InvertedThymidineModification • FluorescenceModification • BiotinModification • DigoxigeninModification • PhosphorylationModification • SpacerModification • AminoandthiolModification 3 AptamerModificationServices

  7. CREATIVEBIOLABSINC. • Anumberoftherapeutic aptamersarecurrentlyevaluated inpreclinicaland clinicaltrials.However,althoughsometimessuccessful,thepracticalapplications ofaptamerare usually muchless efficientandsuccessfulthaninitiallypredicted. As a pioneer and the undisputed global leader in novel drug discovery and manufacturing,CreativeBiolabshasearneda goodreputationinsupporting aptamerdevelopmentandoptimization.Ourprofessionalscientistsare confidentin offering domestic and international customers a range of tailored strategiesand servicestooptimizeaptamers. • OURAPTAMER OPTIMIZATION SERVICESINCLUDE: • Aptamerbasicsequencesoptimization • Aptamerstructurestabilization • Aptamerhydrophobic/hydrophilic portionsoptimization • Chemicalmodifications • SELEXoptimization • Post-SELEXoptimization 4 AptamerOptimizationServices

  8. CREATIVEBIOLABSINC. After several rounds ofscreening bySELEX,theaptamers of interest withtargetmoleculespecificity will be obtained. To ensure theefficiencyoftherapeutic applications, these selected aptamers must be highly stable, of moderate length, and have good pharmacokinetic properties. Therefore, specific assays are needed to characterize and evaluate the properties ofaptamers. Usually,aptamer characterization involves structure determination, binding kineticsmeasurement, andspecificitytest,etc.Based on an advancedtechnologyplatform, ourprofessional scientistsprovidecomprehensiveaptamer characterization servicestoevaluate everyaptamer. 5 Aptamer Characterization Services • OURAPTAMERCHARACTERIZATION SERVICESINCLUDE: • Tertiarystructureanalysis • Bindingaffinitymeasurement • Thermodynamicprofiling • Competitionassays

  9. CREATIVEBIOLABSINC. ServiceFeatures 6 Our Advantages • Extensiveexperienceandadvancedbiotechnologies • Fullycustomizableexperimentaldesign to meetyour R&D timelineandbudget • Best qualitywithcompetitiveprice • Best after-saleservice WhyUs • 10years+of exploration&development • Fullrangeofone-stopservicesforaptamerdevelopment • Specially modified librariesenablethe production ofaptamers with highaffinityandspecificity • Fully customizableprojectprocedure • Yourbest partnerinaptamer-baseddiagnosis andtreatment

  10. ContactUs Quality/Science/Dedication

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