Characterizing the role of IGF-axis on the growth restriction during short photoperiod in Siberian hamster ( Phodopus sungorus ). Ei K. Swe Bio-466H Biological Science Department. Introduction. Photoperiod is an important factor in physiology of temperate animals
Ei K. Swe
Biological Science Department
1) Radioimmunoassay (RIA) for serum level of IGF-I and cortisol
2) Western immunoblot for IGFBPs (specific): IGFBP-1 and IGFBP-3, using rabbit anti-rat IGFBP-1 antiserum and rabbit anti-rat IGFBP-3 antiserum for specific protein
3) RNA-isolation - RNA from liver and muscle tissue by single step method of RNA isolation by acid guanidinuum thiocyanate-phenol chloroform extraction.
4) Northern Blot for mRNA level determination of IGF-I, IGFBP-1 and IGFBP-3 in liver and muscle