Neural Representations of Airflow in Drosophila Mushroom Body
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Neural Representations of Airflow in Drosophila Mushroom Body Akira Mamiya1, Jennifer Beshel1, Chunsu Xu1,2, Yi Zhong1* 1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America, 2 SUNY Stony Brook, Stony Brook, New York, United States of America.

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Neural Representations of Airflow in Drosophila Mushroom Body

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Neural representations of airflow in drosophila mushroom body

Neural Representations of Airflow in Drosophila Mushroom Body

Akira Mamiya1, Jennifer Beshel1, Chunsu Xu1,2, Yi Zhong1*

1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America,

2 SUNY Stony Brook, Stony Brook, New York, United States of America

  • Take home points:

  • Goal: characterize the responses of MB neurons to changes in airflow

  • Method: In vivo calcium imaging from multiple MB regions using genetically altered fruit fly lines and 2-photon microscopy

  • Results:

    • Responses to an airflow stimulus from several sub regions of the MB

    • Different MB sub regions responded differently to different aspects (i.e. on/off responses)

    • Possibly sub sub regions respond differently

    • Dependent on the movement of the 3rd antennal segment suggesting JO involvement


Neural representations of airflow in drosophila mushroom body

  • Method

  • Get TG fruitflies that have a calcium sensor

    • UAS-G-CaMP1.3

    • UAS-G-CaMP1.6

  • Cross with GAL4 fruit fly lines that will allow targeted expression of the calcium sensor in specific cells

    • OK107-Gal4: non-selective MB general

    • c739-Gal4: A/B lobe neurons

    • g0050-Gal4 and c305a-Gal4: A’/B’ lobe neurons

  • Fix fruit fly to an imaging stage

  • Puff air and measure fluorescence:

    • 3s stims

    • 100 ml/min (1.2m/s )

    • 3 min ITI

  • Analyses based on ΔF/Fo which is a measure of changes in Ca++ induced florescence on a pixel by pixel basis


Neural representations of airflow in drosophila mushroom body

Figure 1

Experimental recording sites and raw ΔF/Fo


Neural representations of airflow in drosophila mushroom body

  • Temporal dynamics of responses

    • 1 s data integration window (3 frames)

    • Averaged results

Figure 2


Neural representations of airflow in drosophila mushroom body

Figure 2

Mean On, off responses as a function of lobes

The total area involved in response


Neural representations of airflow in drosophila mushroom body

Figure 3

Different Gal4 lines show that A,B and A’B’ have different response profiles

  • OK107-Gal4: non-selective MB general

  • c739-Gal4: A/B lobe neurons

  • g0050-Gal4 and c305a-Gal4: A’/B’ lobe neurons


Neural representations of airflow in drosophila mushroom body

Figure 4

  • Responses generally depend on movement of the 3rd antennal segment

    • When glued Ca++ responses are greatly diminished


Neural representations of airflow in drosophila mushroom body

Figure 4

  • Responses generally depend on movement of the 3rd antennal segment

    • When glued Ca++ responses are greatly diminished

By comparison with Figure 2 most areas have dropped


Neural representations of airflow in drosophila mushroom body

Figure 5

Sub region specific responses to “on” and “off” phases of the stimulus


Neural representations of airflow in drosophila mushroom body

Figure 5

Watershed Segmentation highlights sub region specific responses


Neural representations of airflow in drosophila mushroom body

Figure 6

“On Off Selectivity Index” (OSI) highlights watershed “patches” within lobes as “on” or “off” response selective (or neither)


Neural representations of airflow in drosophila mushroom body

Figure 7

Off responding patches are spatially organized and stereotypic across individuals


Neural representations of airflow in drosophila mushroom body

Figure 8

Using two Gal4 lines they show that the off and on responses are different subsets of cells


Neural representations of airflow in drosophila mushroom body

Figure 8

g0050-Gal4 cells are significantly more off responsive than c305a-Gal4


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